Figure1. Human CD4 replaces murine CD4. Homozygous CD4 humanized mice display human-CD4 expression in PBMC. No murine CD4 expression is observed. Blood samples from wild-type and homozygous CD4 humanized mice stained with a combination of mAbs (anti-murine CD4, anti-human CD4) and analyzed by flow cytometry.
Figure2. Unaltered immune cell distribution. Homozygous CD4 humanized mice display same frequencies of B cells, NK cells and T cells in spleen as the wildtype mice. Splenocytes are stained with different mAb combinations to assess frequencies of lymphocyte subsets measured by flow cytometry. Grey bars represent values for wild-type mice and hatched bars represent values for CD4 humanized mice.
Figure3. Conserved cytokine expression profile. Homozygous CD4 humanized mice display similar cytokine expression after ConA stimulation. IL-2, IFN-γ and IL-4 concentrations in culture supernatants of mouse splenocytes stimulated with Con A are measured by using a 3-Plex Luminex kit. Black bars represent values for wild-type mice and hatched bars represent data for humanized CD4 mice.
Figure4. Similar Ig production. Homozygous CD4 humanized mice display similar anti-KLH isotyping to wild-type mice. Anti-KLH specific IgG1, and IgG2b polyclonal antibodies are assessed by sandwich ELISA in the serum of mice at day 28 after immunization with KLH and CFA. Serum samples from wild-type (WT) or homozygous CD4 humanized mice (HO CD4) were tested at different dilutions plotted against OD values.