Embryoid Body (EB) Formation Medium

Description:
Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-stage embryos, are pluripotent and have a virtually unlimited capacity of self-renewal. They can differentiate into all cell types comprised of all three embryonic germ layers (ectoderm, mesoderm and endoderm). The formation of embryoid bodies (EBs) is a principal step in the differentiation of ESCs.  When maintained in the EB formation medium and in the absence of MEF feeder layers, ES cells differentiate spontaneously and form three-dimensional aggregates. This structure facilitates multicellular interactions in which cell-cell contact occurs and gap junctions are established.

Highlights:
- Highly effective in inducing human and mouse embryonic stem cells to form embryoid bodies (EB).

- Batch-to-batch consistency.

Kit Components:

Stability/Storage:
- All products should be stored in the dark.

- Embryoid Body Formation Basal Medium is stable at 2-8^oC for up to one year; other components are stable at -20^oC for up to two years.

- Products should be discarded beyond the labeled expiration date.

- Once prepared, the fully supplemented (complete) medium can be stored for up to one month when stored in the dark at 2-8^oC.

- For optimal performance, repeated warm-cooling and freeze-thawing should be avoided.

Quality Control:
Tested for perfomance on EB formation.  The standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin

Intended Use:

Intended for non-human laboratory research use only.