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Embryoid Body (EB) Formation Medium

None Embryoid Body (EB) Formation Medium MUXES-90051
Catalog Number:
MUXES-90051
Specification:
価格:
Description: Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-

Description:
Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-stage embryos, are pluripotent and have a virtually unlimited capacity of self-renewal. They can differentiate into all cell types comprised of all three embryonic germ layers (ectoderm, mesoderm and endoderm). The formation of embryoid bodies (EBs) is a principal step in the differentiation of ESCs.  When maintained in the EB formation medium and in the absence of MEF feeder layers, ES cells differentiate spontaneously and form three-dimensional aggregates. This structure facilitates multicellular interactions in which cell-cell contact occurs and gap junctions are established.

Highlights:
- Highly effective in inducing human and mouse embryonic stem cells to form embryoid bodies (EB)
.

- Batch-to-batch consistency.

Kit Components:

 Embryoid Body (EB) Formation Basal
 Medium
 435 mL
 Embryoid Body (EB) Formation-
  Qualified Fetal Bovine Serum
 50 mL
 Penicillin-Streptomycin  5 mL
 Glutamine  5 mL
 Non-Essential Amino Acid  5 mL
 2-Mercaptoethanol  500 μl

 

Stability/Storage:
- All products should be stored in the dark.

- Embryoid Body Formation Basal Medium is stable at 2-8oC for up to one year; other components are stable at -20oC for up to two years.

- Products should be discarded beyond the labeled expiration date.

- Once prepared, the fully supplemented (complete) medium can be stored for up to one month when stored in the dark at 2-8oC.

- For optimal performance, repeated warm-cooling and freeze-thawing should be avoided.

Quality Control:
Tested for perfomance on EB formation.
  The standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin

 

Intended Use:

Intended for non-human laboratory research use only.