Alox15-flox Mouse
一般名
Alox15-flox
製品ID
S-CKO-01155
背景情報
C57BL/6JCya
系統ID
CKOCMP-11687-Alox15-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Alox15-flox Mouse（カタログ番号S-CKO-01155）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Alox15-flox
系統ID
CKOCMP-11687-Alox15-B6J-VA
遺伝子名
製品ID
S-CKO-01155
遺伝子別名
12-LO, 15-LOX, L-12LO, Alox12l, 12/15-LO
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000019068
NCBIトランスクリプトID
NM_009660
ターゲット領域
Exon 2
有効領域の大きさ
~0.6 kb
遺伝子研究の概要
Alox15, also known as 15-lipoxygenase-1 or 12/15-lipoxygenase (12/15-LOX), is an enzyme that oxidizes polyunsaturated fatty acids, generating bioactive lipid metabolites. It is involved in oxidative and inflammatory responses, and its metabolites play roles in gene expression and cytokine production related to inflammation and its resolution [2].
Myocardial-specific knockout of Alox15 in mice alleviated myocardial ischemia-reperfusion (I/R) injury, restored cardiac function, and reduced cardiomyocyte ferroptosis. 15-Hydroperoxyeicosatetraenoic acid (15-HpETE), an Alox15-derived metabolite, was identified as a trigger for cardiomyocyte ferroptosis [1].
Intestinally-targeted transgenic expression of ALOX15 in mice inhibited dextran sodium sulfate-induced colitis from promoting azoxymethane-induced colorectal tumorigenesis, demonstrating its role in suppressing inflammation-driven colorectal tumorigenesis [3].
In asthma, silencing ALOX15 in HDM/LPS-stimulated 16HBE cells decreased ferroptosis, indicating its role in asthma-related ferroptosis [4].
Macrophage-derived exosomes with miRNA-660-5p attenuated ALOX15 expression in cervical cancer cells to suppress ferroptosis, and ALOX15 expression was positively associated with good prognosis in cervical cancer [5].
Electroacupuncture relieved neuropathic pain by suppressing ferroptosis in the dorsal root ganglion via the SAT1/ALOX15 signaling pathway [6].
DHODH inhibited ALOX15 expression by inhibiting P53, reducing neuronal ferroptosis after spinal cord injury [7].
In conclusion, Alox15 is crucial in processes like inflammation, ferroptosis, and disease development. Gene-knockout mouse models have significantly contributed to understanding its role in diseases such as myocardial I/R injury, colorectal cancer, asthma, cervical cancer, neuropathic pain, and spinal cord injury. These studies offer potential therapeutic targets for treating these conditions.
References：
1. Cai, Wenbin, Liu, Le, Shi, Xuelian, Zhu, Yi, Zhang, Xu. 2023. Alox15/15-HpETE Aggravates Myocardial Ischemia-Reperfusion Injury by Promoting Cardiomyocyte Ferroptosis. In Circulation, 147, 1444-1460. doi:10.1161/CIRCULATIONAHA.122.060257. https://pubmed.ncbi.nlm.nih.gov/36987924/
2. Singh, Nikhlesh K, Rao, Gadiparthi N. 2018. Emerging role of 12/15-Lipoxygenase (ALOX15) in human pathologies. In Progress in lipid research, 73, 28-45. doi:10.1016/j.plipres.2018.11.001. https://pubmed.ncbi.nlm.nih.gov/30472260/
3. Tian, Rui, Zuo, Xiangsheng, Jaoude, Jonathan, Colby, Jennifer, Shureiqi, Imad. 2017. ALOX15 as a suppressor of inflammation and cancer: Lost in the link. In Prostaglandins & other lipid mediators, 132, 77-83. doi:10.1016/j.prostaglandins.2017.01.002. https://pubmed.ncbi.nlm.nih.gov/28089732/
4. Zhang, Weizhen, Huang, Fangfang, Ding, Xuexuan, Wang, Wenjian, Luo, Lianxiang. 2024. Identifying ALOX15-initiated lipid peroxidation increases susceptibility to ferroptosis in asthma epithelial cells. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167176. doi:10.1016/j.bbadis.2024.167176. https://pubmed.ncbi.nlm.nih.gov/38641013/
5. Luo, Yanlin, Chen, Yibing, Jin, Huan, Liu, Quentin, Zou, Zhengzhi. 2023. The suppression of cervical cancer ferroptosis by macrophages: The attenuation of ALOX15 in cancer cells by macrophages-derived exosomes. In Acta pharmaceutica Sinica. B, 13, 2645-2662. doi:10.1016/j.apsb.2023.03.025. https://pubmed.ncbi.nlm.nih.gov/37425043/
6. Wan, Kexing, Jia, Min, Zhang, Hong, Liu, Yongmin, Li, Man. 2023. Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling. In Molecular neurobiology, 60, 6121-6132. doi:10.1007/s12035-023-03463-z. https://pubmed.ncbi.nlm.nih.gov/37421564/
7. Li, Dachuan, Lu, Xiao, Xu, Guangyu, Zou, Fei, Ma, Xiaosheng. 2023. Dihydroorotate dehydrogenase regulates ferroptosis in neurons after spinal cord injury via the P53-ALOX15 signaling pathway. In CNS neuroscience & therapeutics, 29, 1923-1939. doi:10.1111/cns.14150. https://pubmed.ncbi.nlm.nih.gov/36942513/
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