Ddx6-flox Mouse
一般名
Ddx6-flox
製品ID
S-CKO-02024
背景情報
C57BL/6JCya
系統ID
CKOCMP-13209-Ddx6-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ddx6-flox Mouse（カタログ番号S-CKO-02024）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ddx6-flox
系統ID
CKOCMP-13209-Ddx6-B6J-VA
遺伝子名
製品ID
S-CKO-02024
遺伝子別名
p54, rck, HLR2, mRCK/P54, 1110001P04Rik, C430015D01Rik, E230023J21Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 9
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000170489
NCBIトランスクリプトID
NM_001110826
ターゲット領域
Exon 3~4
有効領域の大きさ
~2.1 kb
遺伝子研究の概要
Ddx6, also known as Rck/p54, is a member of the DEAD-box family of helicases highly conserved from unicellular eukaryotes to vertebrates. It is an essential component of cytoplasmic RNA-processing bodies (P-bodies) and plays a crucial role in multiple post-transcriptional processes such as mRNA storage, translational repression, and decay [3]. It is also involved in pathways like miRNA-mediated gene silencing, alternative translation initiation, and RNA editing [3,7]. In addition, Ddx6 has been implicated in the regulation of cell fate transitions, stress granule and P-body assembly, and immune-related regulatory networks, making it a key player in maintaining cellular homeostasis [1,2,4]. Genetic models, especially knockout (KO) and conditional knockout (CKO) mouse models, are valuable tools for studying its functions.
Suppression of Ddx6 in human and mouse primed embryonic stem cells endows them with a differentiation-resistant, “hyper-pluripotent” state and enables easy reprogramming to a naive state [1]. In adult progenitors, Ddx6 controls the balance between self-renewal and differentiation in a context-dependent manner [1]. Ddx6-silencing in non-immune cells suppresses the NF-κB pathway and inhibits activation of the IL-6 amplifier, while its overexpression enhances NF-κB promoter activity, suggesting its involvement in the pathogenesis of inflammatory diseases [5]. In pancreatic cancer, compared with adjacent tissues, Ddx6 expression is abnormally increased in human pancreatic cancer tissues, and overexpression promotes cancer cell proliferation and tumor formation, while knockdown has the opposite effects [6].
In conclusion, Ddx6 is a multifunctional regulator in post-transcriptional gene expression. Studies using KO/CKO mouse models have revealed its significance in cell fate determination, stress response, and the pathogenesis of inflammatory and cancerous diseases. Understanding Ddx6's functions provides insights into biological processes and may offer potential therapeutic targets for related diseases.
References：
1. Di Stefano, Bruno, Luo, En-Ching, Haggerty, Chuck, Yeo, Gene W, Hochedlinger, Konrad. 2019. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. In Cell stem cell, 25, 622-638.e13. doi:10.1016/j.stem.2019.08.018. https://pubmed.ncbi.nlm.nih.gov/31588046/
2. Ripin, Nina, Macedo de Vasconcelos, Luisa, Ugay, Daniella A, Parker, Roy. 2024. DDX6 modulates P-body and stress granule assembly, composition, and docking. In The Journal of cell biology, 223, . doi:10.1083/jcb.202306022. https://pubmed.ncbi.nlm.nih.gov/38536035/
3. Ostareck, Dirk H, Naarmann-de Vries, Isabel S, Ostareck-Lederer, Antje. 2014. DDX6 and its orthologs as modulators of cellular and viral RNA expression. In Wiley interdisciplinary reviews. RNA, 5, 659-78. doi:10.1002/wrna.1237. https://pubmed.ncbi.nlm.nih.gov/24788243/
4. Wiley, Mandi M, Khatri, Bhuwan, Joachims, Michelle L, Nordmark, Gunnel, Lessard, Christopher J. 2023. Variants in the DDX6-CXCR5 autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.10.05.561076. https://pubmed.ncbi.nlm.nih.gov/39071447/
5. Naito, Seiichiro, Tanaka, Hiroki, Jiang, Jing-Jing, Hashimoto, Shigeru, Murakami, Masaaki. 2024. DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation. In Biochemical and biophysical research communications, 703, 149666. doi:10.1016/j.bbrc.2024.149666. https://pubmed.ncbi.nlm.nih.gov/38377944/
6. Deng, Xin, Liu, Zhen, Wang, Baosheng, Ma, Jia, Meng, Xiangpeng. . The DDX6/KIFC1 signaling axis, as regulated by YY1, contributes to the malignant behavior of pancreatic cancer. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, e23581. doi:10.1096/fj.202400166R. https://pubmed.ncbi.nlm.nih.gov/38551642/
7. Shih, Chia-Yu, Chen, Yun-Chi, Lin, Heng-Yi, Chu, Chia-Ying. 2023. RNA Helicase DDX6 Regulates A-to-I Editing and Neuronal Differentiation in Human Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms24043197. https://pubmed.ncbi.nlm.nih.gov/36834609/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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