Snai2-flox Mouse
一般名
Snai2-flox
製品ID
S-CKO-05128
背景情報
C57BL/6JCya
系統ID
CKOCMP-20583-Snai2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Snai2-flox Mouse（カタログ番号S-CKO-05128）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Snai2-flox
系統ID
CKOCMP-20583-Snai2-B6J-VA
遺伝子名
製品ID
S-CKO-05128
遺伝子別名
Slug, Slugh, Snail2
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 16
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000023356
NCBIトランスクリプトID
NM_011415
ターゲット領域
Exon 2~3
有効領域の大きさ
~3.7 kb
遺伝子研究の概要
Snai2, also known as Slug, is an evolutionarily conserved C2H2 zinc finger protein encoded by the SNAI2 gene. It functions as a transcription factor, initially recognized for its role in epithelial-to-mesenchymal transition (EMT), a process involved in tissue development and tumorigenesis. It also participates in various biological processes such as tumor metastasis, stem cell biology, cellular differentiation, vascular remodeling, and DNA damage repair by facilitating epigenetic regulation of transcriptional programs [2].
In mice, hepatocyte-specific deletion of both Snai1 and Snai2 suppresses liver cyclin A2/D1 expression and regenerative hepatocyte proliferation after hepatectomy or CCl4 treatments but augments CCl4-stimulated HSC activation and liver fibrosis, indicating that Snai2 promotes liver regeneration and suppresses liver fibrosis [1]. In rhabdomyosarcoma (RMS), SNAI2 is abnormally expressed, promoting proliferation, metastasis, and vasculogenic mimicry. The deubiquitinase USP7 and E3 ligase TRIM21 balance SNAI2 homeostasis, which is crucial for RMS progression [3]. In RMS, SNAI2 also maintains NOTCH1 expression through interaction with CTCF, and its suppression of apoptosis post-radiation is independent of NOTCH1 effects on self-renewal and differentiation [4]. Additionally, SNAI2-mediated repression of BIM protects RMS from ionizing radiation [5]. In oral leukoplakia, SNAI2 promotes malignant transformation by modulating partial epithelial-mesenchymal transition (p-EMT) [6].
In conclusion, Snai2 is a key regulator in multiple biological processes and diseases. Studies using gene knockout (KO) or conditional knockout (CKO) mouse models have revealed its significance in liver regeneration and fibrosis, as well as in cancer-related processes like those in rhabdomyosarcoma and oral leukoplakia. These models provide valuable insights into the role of Snai2, helping to understand the underlying mechanisms of these diseases and potentially guiding the development of new therapeutic strategies.
References：
1. Wang, Pingping, Kang, Qianqian, Wu, Wen-Shu, Rui, Liangyou. 2024. Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice. In Cell reports, 43, 113875. doi:10.1016/j.celrep.2024.113875. https://pubmed.ncbi.nlm.nih.gov/38451818/
2. Zhou, Wenhui, Gross, Kayla M, Kuperwasser, Charlotte. 2019. Molecular regulation of Snai2 in development and disease. In Journal of cell science, 132, . doi:10.1242/jcs.235127. https://pubmed.ncbi.nlm.nih.gov/31792043/
3. Zhang, Ruyue, Zhang, Daidi, Han, Fugen, Zhu, Qingwen, Qin, Yanru. 2024. The deubiquitinase USP7 and E3 ligase TRIM21 regulate vasculogenic mimicry and malignant progression of RMS by balancing SNAI2 homeostasis. In Journal of experimental & clinical cancer research : CR, 43, 135. doi:10.1186/s13046-024-03056-1. https://pubmed.ncbi.nlm.nih.gov/38702792/
4. Sreenivas, Prethish, Wang, Long, Wang, Meng, Stanton, Benjamin Z, Ignatius, Myron S. 2023. A SNAI2/CTCF Interaction is Required for NOTCH1 Expression in Rhabdomyosarcoma. In Molecular and cellular biology, 43, 547-565. doi:10.1080/10985549.2023.2256640. https://pubmed.ncbi.nlm.nih.gov/37882064/
5. Wang, Long, Hensch, Nicole R, Bondra, Kathryn, Houghton, Peter J, Ignatius, Myron S. 2021. SNAI2-Mediated Repression of BIM Protects Rhabdomyosarcoma from Ionizing Radiation. In Cancer research, 81, 5451-5463. doi:10.1158/0008-5472.CAN-20-4191. https://pubmed.ncbi.nlm.nih.gov/34462275/
6. Shang, Qianhui, Peng, Jiakuan, Jiang, Yuchen, Xu, Hao, Chen, Qianming. 2022. SNAI2 promotes the malignant transformation of oral leukoplakia by modulating p-EMT. In Oral diseases, 29, 3232-3242. doi:10.1111/odi.14321. https://pubmed.ncbi.nlm.nih.gov/35894087/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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