Cmpk2-flox Mouse
一般名
Cmpk2-flox
製品ID
S-CKO-06500
背景情報
C57BL/6JCya
系統ID
CKOCMP-22169-Cmpk2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Cmpk2-flox Mouse（カタログ番号S-CKO-06500）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cmpk2-flox
系統ID
CKOCMP-22169-Cmpk2-B6J-VA
遺伝子名
製品ID
S-CKO-06500
遺伝子別名
TDKI, Tyki, 1200004E04Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 12
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000020969
NCBIトランスクリプトID
NM_020557
ターゲット領域
Exon 2
有効領域の大きさ
~1.4 kb
遺伝子研究の概要
Cmpk2, short for cytidine/uridine monophosphate kinase 2, is an essential mitochondria-associated gene. It regulates mtDNA replication and is involved in multiple pathways, such as the NLRP3 inflammasome-related inflammatory pathway [1,2,3,6,7,8]. It also has antiviral functions against various viruses including Zika virus, flaviviruses, and coronaviruses [5,9]. Its expression is associated with many biological processes and disease conditions, making it an important target for research.
In ischemic stroke, microglia/macrophage Cmpk2 knockdown using Cre recombination-dependent adeno-associated virus suppresses brain inflammatory responses, reduces infarcts, and improves neurological outcomes. It limits newly synthesized mtDNA and Ox-mtDNA formation, blocking NLRP3 inflammasome activation [1].
In spinal cord injury rats, AAV-mediated Cmpk2 knockdown or electroacupuncture-mediated Cmpk2 down-regulation inhibits NLRP3 activation, improves motor function [2].
In sepsis, dracorhodin, a Cmpk2 inhibitor, exerts anti-inflammatory effects by regulating the NLRP3 inflammasome via the LPS-induced Cmpk2 pathway [3].
In mice, homozygous Cmpk2-KO mice develop progressive calcification in the thalamus region, mimicking the brain calcification pathology in patients with biallelic CMPK2 variants [4].
In atherosclerosis mouse models, EC-GATA6 deletion decreases monocyte recruitment and atherosclerotic lesion formation through regulation of the CMPK2-Nlrp3 pathway [6].
In glucocorticoid-induced osteoporosis study, inhibiting Cmpk2 expression in preosteoblasts alleviates glucocorticoid-induced cellular senescence and promotes osteogenic differentiation [7].
In conclusion, Cmpk2 plays a crucial role in regulating mitochondrial function, inflammation, and antiviral responses. Gene knockout and conditional knockout mouse models have revealed its significant contributions in diseases such as ischemic stroke, spinal cord injury, sepsis, brain calcification, atherosclerosis, and glucocorticoid-induced osteoporosis, providing potential therapeutic targets for these conditions.
References：
1. Guan, Xin, Zhu, Sitong, Song, Jinqian, Xu, Xiaojun, Pang, Tao. 2024. Microglial CMPK2 promotes neuroinflammation and brain injury after ischemic stroke. In Cell reports. Medicine, 5, 101522. doi:10.1016/j.xcrm.2024.101522. https://pubmed.ncbi.nlm.nih.gov/38701781/
2. Chen, Yi, Wu, Lei, Shi, Mengting, Shao, XiaoMei, Ma, Ruijie. 2022. Electroacupuncture Inhibits NLRP3 Activation by Regulating CMPK2 After Spinal Cord Injury. In Frontiers in immunology, 13, 788556. doi:10.3389/fimmu.2022.788556. https://pubmed.ncbi.nlm.nih.gov/35401582/
3. Zhang, Wendan, Jiang, Honghong, Huang, Pengli, Liu, Sanhong, Zhang, Weidong. . Dracorhodin targeting CMPK2 attenuates inflammation: A novel approach to sepsis therapy. In Clinical and translational medicine, 13, e1449. doi:10.1002/ctm2.1449. https://pubmed.ncbi.nlm.nih.gov/37859535/
4. Zhao, Miao, Su, Hui-Zhen, Zeng, Yi-Heng, Cheng, Xuewen, Chen, Wan-Jin. 2022. Loss of function of CMPK2 causes mitochondria deficiency and brain calcification. In Cell discovery, 8, 128. doi:10.1038/s41421-022-00475-2. https://pubmed.ncbi.nlm.nih.gov/36443312/
5. Pawlak, Joanna B, Hsu, Jack Chun-Chieh, Xia, Hongjie, Cresswell, Peter, Laurent-Rolle, Maudry. 2023. CMPK2 restricts Zika virus replication by inhibiting viral translation. In PLoS pathogens, 19, e1011286. doi:10.1371/journal.ppat.1011286. https://pubmed.ncbi.nlm.nih.gov/37075076/
6. Wu, Wenrun, Bao, Wenzhen, Chen, Xiaoli, Zhang, Yuzhen, Zhuang, Tao. 2023. Endothelial Gata6 deletion reduces monocyte recruitment and proinflammatory macrophage formation and attenuates atherosclerosis through Cmpk2-Nlrp3 pathways. In Redox biology, 64, 102775. doi:10.1016/j.redox.2023.102775. https://pubmed.ncbi.nlm.nih.gov/37339559/
7. Cao, Nianping, Wang, Zhihang, Huang, Chongjun, Xu, Ying, Tian, Ye. 2023. Cmpk2 regulates mitochondrial function in glucocorticoid-induced osteoblast senescence and affects glucocorticoid-inhibited osteoblast differentiation. In Archives of gerontology and geriatrics, 114, 105080. doi:10.1016/j.archger.2023.105080. https://pubmed.ncbi.nlm.nih.gov/37269696/
8. Jin, Li, Chen, Qiyue, Hu, Ke, Qi, Weizhong, Yu, Qinghong. 2024. The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation. In International journal of biological sciences, 20, 1617-1633. doi:10.7150/ijbs.90677. https://pubmed.ncbi.nlm.nih.gov/38481810/
9. Zhu, Mingjun, Lv, Jiahuang, Wang, Wei, Ding, Siyuan, Li, Bin. 2023. CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner. In PLoS biology, 21, e3002039. doi:10.1371/journal.pbio.3002039. https://pubmed.ncbi.nlm.nih.gov/36930652/
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