Il22ra2-flox Mouse
一般名
Il22ra2-flox
製品ID
S-CKO-07915
背景情報
C57BL/6JCya
系統ID
CKOCMP-237310-Il22ra2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Il22ra2-flox Mouse（カタログ番号S-CKO-07915）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Il22ra2-flox
系統ID
CKOCMP-237310-Il22ra2-B6J-VA
遺伝子名
製品ID
S-CKO-07915
遺伝子別名
CRF2X, CRF2-10, CRF2-s1, Il-22bp
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 10
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000036564
NCBIトランスクリプトID
NM_178258
ターゲット領域
Exon 2
有効領域の大きさ
~1.5 kb
遺伝子研究の概要
Il22ra2, encoding the IL-22 binding protein (IL-22BP), is a gene with significant biological importance. IL-22BP is a soluble decoy that decreases the bioavailability of interleukin 22 (IL-22), thus attenuating IL-22 signaling. IL-22 is involved in promoting intestinal barrier integrity and stimulating epithelial cells to defend against enteric infections, so Il22ra2 indirectly impacts these processes [1].
In gene knockout studies, Il22ra2-/-mice show enhanced protection against Clostridioides difficile and Citrobacter rodentium infections. This protection is due to IL-22-induced antimicrobial mechanisms before infection, and the gut microbiota of these knockout mice can mitigate infection in wild-type mice upon transfer. Resistance to C. difficile infection is related to increased acetate production by Il22ra2-/-associated microbiota [1].
In the context of multiple sclerosis, a rare coding variant rs28385692 in the IL22RA2 signal peptide coding region decreases the secretion of IL-22BP isoforms and is associated with an augmented risk for the disease [2]. Also, Il22ra2-/-mice have a less severe course of experimental autoimmune encephalomyelitis, with less demyelination and immune cell infiltration in the central nervous system, suggesting that lack of IL-22BP leads to higher IL-22 availability which has a protective effect in CNS inflammation [3].
In pneumococcal pneumonia, Il22ra2 deficiency favors down-regulation of oxidative phosphorylation genes in an IL-22-dependent manner, and Il22ra2-/-mice are more resistant to the infection [4].
In the bleomycin model of acute lung injury, Il22ra2-/-mice have greater sensitivity and pulmonary inflammation in the acute phase, driven by excess IL-22 production and the influx of pathogenic IL-17A+ γδ T cells to the lung [5].
In conclusion, Il22ra2 plays a crucial role in modulating IL-22 bioavailability and subsequent signaling, influencing host defense mechanisms. Gene knockout mouse models have revealed its significance in various disease areas such as enteric infections, multiple sclerosis, neuroinflammation, pneumococcal pneumonia, and acute lung injury. Understanding Il22ra2 can provide insights into disease mechanisms and potential therapeutic strategies.
References：
1. Fachi, José L, Di Luccia, Blanda, Gilfillan, Susan, Gordon, Jeffrey I, Colonna, Marco. 2024. Deficiency of IL-22-binding protein enhances the ability of the gut microbiota to protect against enteric pathogens. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2321836121. doi:10.1073/pnas.2321836121. https://pubmed.ncbi.nlm.nih.gov/38687788/
2. Gómez-Fernández, Paloma, Lopez de Lapuente Portilla, Aitzkoa, Astobiza, Ianire, Lill, Christina M, Vandenbroeck, Koen. 2020. The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis. In Cells, 9, . doi:10.3390/cells9010175. https://pubmed.ncbi.nlm.nih.gov/31936765/
3. Laaksonen, H, Guerreiro-Cacais, A O, Adzemovic, M Z, Jagodic, M, Olsson, T. 2014. The multiple sclerosis risk gene IL22RA2 contributes to a more severe murine autoimmune neuroinflammation. In Genes and immunity, 15, 457-65. doi:10.1038/gene.2014.36. https://pubmed.ncbi.nlm.nih.gov/25008863/
4. Trevejo-Nunez, Giraldina, Elsegeiny, Waleed, Aggor, Felix E Y, Kolls, Jay K, Gaffen, Sarah L. 2019. Interleukin-22 (IL-22) Binding Protein Constrains IL-22 Activity, Host Defense, and Oxidative Phosphorylation Genes during Pneumococcal Pneumonia. In Infection and immunity, 87, . doi:10.1128/IAI.00550-19. https://pubmed.ncbi.nlm.nih.gov/31451621/
5. Zhang, Zhe, Chakawa, Mazvita B, Galeas-Pena, Michelle, Jones, MaryJane, Pociask, Derek. 2023. IL-22 Binding Protein Controls IL-22-Driven Bleomycin-Induced Lung Injury. In The American journal of pathology, 194, 338-352. doi:10.1016/j.ajpath.2023.11.011. https://pubmed.ncbi.nlm.nih.gov/38101567/
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