Dkc1-flox Mouse
一般名
Dkc1-flox
製品ID
S-CKO-08670
背景情報
C57BL/6JCya
系統ID
CKOCMP-245474-Dkc1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Dkc1-flox Mouse（カタログ番号S-CKO-08670）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Dkc1-flox
系統ID
CKOCMP-245474-Dkc1-B6J-VA
遺伝子名
製品ID
S-CKO-08670
遺伝子別名
--
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr X
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000033776
NCBIトランスクリプトID
NM_001030307
ターゲット領域
Exon 4~5
有効領域の大きさ
~1.0 kb
遺伝子研究の概要
Dkc1, also known as dyskerin pseudouridine synthase 1, is associated with the formation of certain small RNAs and telomerase activity [5]. Mutations in DKC1 can lead to dyskeratosis congenita, a rare hereditary disease [3]. In normal biological processes, it may be involved in pathways related to cell proliferation, ribosome biogenesis, and telomere maintenance.
DKC1 has been found to play a significant role in multiple cancers. In colorectal cancer, DKC1 promotes cell proliferation by binding to and stabilizing the mRNA of several ribosomal proteins, and its knockdown attenuates cell growth. Dual inhibition of DKC1 and MEK1/2 synergistically restrains colorectal cancer cell growth [1].
In pancreatic ductal adenocarcinoma, SENP3 interacts with DKC1 and catalyzes its deSUMOylation, causing DKC1 instability and impairing cell migration. Overexpression of DKC1 abates the anti-metastasis effect of SENP3 [2].
In lung adenocarcinoma, DKC1 is overexpressed, and its knockdown induces G1 phase arrest, inhibits cell proliferation, and leads to telomere-related senescence and apoptosis [4].
In glioma, DKC1 expression is increased, and its knockdown inhibits cell growth, migration, and invasion [5].
In gastric cancer, DKC1 aggravates cell migration and invasion through up-regulating the expression of TNFAIP6 [6].
In neuroblastoma, DKC1 is highly expressed, and its knockout decreases cell activity, proliferation, invasion, and migration while increasing apoptosis [7]. A meta-analysis also shows that high DKC1 expression is predictive of worse prognosis and poorer clinicopathological parameters in malignancies [8].
In conclusion, Dkc1 is crucial for normal cellular functions, especially those related to ribosome production and telomerase complex function. Its dysregulation significantly impacts the development and progression of various cancers, such as colorectal, pancreatic, lung, glioma, gastric, and neuroblastoma cancers. The findings from loss-of-function experiments in these contexts have enhanced our understanding of Dkc1's role in disease, suggesting it could be a potential therapeutic target.
References：
1. Kan, Guangyan, Wang, Ziyang, Sheng, Chunjie, Mao, Yizhi, Chen, Shuai. 2021. Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8, 2004344. doi:10.1002/advs.202004344. https://pubmed.ncbi.nlm.nih.gov/34026451/
2. Wu, Xiao, Li, Jian-Hui, Xu, Long, Ni, Xuhao, Yin, Xiao-Yu. 2023. SUMO specific peptidase 3 halts pancreatic ductal adenocarcinoma metastasis via deSUMOylating DKC1. In Cell death and differentiation, 30, 1742-1756. doi:10.1038/s41418-023-01175-4. https://pubmed.ncbi.nlm.nih.gov/37188742/
3. AlSabbagh, Manahel Mahmood. 2020. Dyskeratosis congenita: a literature review. In Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, 18, 943-967. doi:10.1111/ddg.14268. https://pubmed.ncbi.nlm.nih.gov/32930426/
4. Kan, Guangyan, Wang, Ziyang, Sheng, Chunjie, Mao, Yizhi, Chen, Shuai. 2021. Inhibition of DKC1 induces telomere-related senescence and apoptosis in lung adenocarcinoma. In Journal of translational medicine, 19, 161. doi:10.1186/s12967-021-02827-0. https://pubmed.ncbi.nlm.nih.gov/33879171/
5. Miao, Fa-An, Chu, Kun, Chen, Hai-Rong, Bai, Jin, You, Yong-Ping. 2019. Increased DKC1 expression in glioma and its significance in tumor cell proliferation, migration and invasion. In Investigational new drugs, 37, 1177-1186. doi:10.1007/s10637-019-00748-w. https://pubmed.ncbi.nlm.nih.gov/30847721/
6. Chen, Huihua, Wu, Yibo, Jiang, Yancheng, Chen, Zixuan, Zheng, Tingjin. 2024. DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6. In Functional & integrative genomics, 24, 38. doi:10.1007/s10142-024-01313-2. https://pubmed.ncbi.nlm.nih.gov/38376551/
7. Wang, Xiao-Hui, Zhang, Shu-Feng, Wu, Hai-Ying, Wang, Xu-Hui, Gao, Tian-Hui. 2023. miRNA326-5p Targets DKC1 Gene to Regulate Apoptosis-Related Proteins and Intervene in the Development of Neuroblastoma. In Analytical cellular pathology (Amsterdam), 2023, 6761894. doi:10.1155/2023/6761894. https://pubmed.ncbi.nlm.nih.gov/37426487/
8. Zhang, Qin, Liu, Xiaohan, Zou, Zhenhong, Zhou, Bin. 2023. Evidence from a meta-analysis for the prognostic and clinicopathological importance of DKC1 in malignancies. In Future oncology (London, England), 19, 473-484. doi:10.2217/fon-2022-1125. https://pubmed.ncbi.nlm.nih.gov/36876511/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。