Cyp39a1-flox Mouse
一般名
Cyp39a1-flox
製品ID
S-CKO-11933
背景情報
C57BL/6NCya
系統ID
CKOCMP-56050-Cyp39a1-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Cyp39a1-flox Mouse（カタログ番号S-CKO-11933）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cyp39a1-flox
系統ID
CKOCMP-56050-Cyp39a1-B6N-VA
遺伝子名
製品ID
S-CKO-11933
遺伝子別名
mCYP39A1
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conditional knockout
染色体
Chr 17
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000170988
NCBIトランスクリプトID
NM_018887
ターゲット領域
Exon 3
有効領域の大きさ
~0.7 kb
遺伝子研究の概要
CYP39A1, a member of the cytochrome P450 family, functions as an oxysterol 7α -hydroxylase, selectively catalyzing the 7α -hydroxylation of 24 -hydroxycholesterol [8,9]. It is involved in the alternative bile acid synthesis pathway in the liver and is also expressed in the ciliary nonpigmented epithelium of the eye, suggesting a role in ocular sterol metabolism [9]. It may be regulated by the orphan nuclear receptor RORα, and abnormal expression can lead to the accumulation of 24S -OHC, influencing neurodegenerative disease-related deterioration [7].
In hepatocellular carcinoma (HCC), CYP39A1 protein and mRNA expression are downregulated compared to non-cancerous liver tissues. Overexpression of CYP39A1 inhibits HepG2 and SMMC-7721 cell viability, while knockout promotes HCC cell growth, indicating it may act as a tumor suppressor gene [1].
In exfoliation syndrome, carriers of functionally deficient CYP39A1 mutations have a significantly increased risk of glaucoma and blindness, and those with the CYP39A1 G204E mutation have a higher incidence of exfoliation glaucoma and more severe glaucoma [2,3].
In high-altitude pulmonary edema (HAPE), the methylation level of CYP39A1 is associated with the risk of HAPE in the Chinese population, with certain CpG sites having either a protective or risk-increasing role [4].
In advanced head and neck cancer, the CYP39A1 rs7761731 polymorphism is associated with a higher incidence of leucopenia and infections or death during intensive induction chemotherapy [5].
In cholangiocarcinoma, low CYP39A1 expression is correlated with metastasis and poor prognosis [6].
In conclusion, CYP39A1 plays diverse roles in multiple biological processes and disease conditions. Its downregulation in HCC, association with glaucoma in exfoliation syndrome, and link to HAPE risk, among others, highlight its significance. Gene knockout and related functional studies in various models contribute to understanding its role in these diseases, potentially providing new directions for disease diagnosis, prevention, and treatment [1-6].
References：
1. Li, Dan, Yu, Tao, Hu, Junjie, Gu, Lijuan, Zeng, Zhi. 2021. Downregulation of CYP39A1 Serves as a Novel Biomarker in Hepatocellular Carcinoma with Worse Clinical Outcome. In Oxidative medicine and cellular longevity, 2021, 5175581. doi:10.1155/2021/5175581. https://pubmed.ncbi.nlm.nih.gov/35003516/
2. Bell, Katharina, Ozaki, Mineo, Mori, Kazuhiko, Khor, Chiea Chuen, Aung, Tin. 2021. Association of the CYP39A1 G204E Genetic Variant with Increased Risk of Glaucoma and Blindness in Patients with Exfoliation Syndrome. In Ophthalmology, 129, 406-413. doi:10.1016/j.ophtha.2021.11.001. https://pubmed.ncbi.nlm.nih.gov/34763023/
3. Li, Zheng, Wang, Zhenxun, Lee, Mei Chin, Tam, Wai Leong, Khor, Chiea Chuen. . Association of Rare CYP39A1 Variants With Exfoliation Syndrome Involving the Anterior Chamber of the Eye. In JAMA, 325, 753-764. doi:10.1001/jama.2021.0507. https://pubmed.ncbi.nlm.nih.gov/33620406/
4. Wang, Pingyi, Lu, Hongyan, Rong, Hao, He, Yongjun, Jin, Tianbo. 2023. The Association of Methylation Level in the CYP39A1 Gene with High Altitude Pulmonary Edema in the Chinese Population. In Pharmacogenomics and personalized medicine, 16, 617-628. doi:10.2147/PGPM.S397862. https://pubmed.ncbi.nlm.nih.gov/37366513/
5. Melchardt, Thomas, Hufnagl, Clemens, Magnes, Teresa, Greil, Richard, Egle, Alexander. 2015. CYP39A1 polymorphism is associated with toxicity during intensive induction chemotherapy in patients with advanced head and neck cancer. In BMC cancer, 15, 725. doi:10.1186/s12885-015-1776-x. https://pubmed.ncbi.nlm.nih.gov/26475344/
6. Khenjanta, Chakkaphan, Thanan, Raynoo, Jusakul, Apinya, Pairojkul, Chawalit, Yongvanit, Puangrat. . Association of CYP39A1, RUNX2 and oxidized alpha-1 antitrypsin expression in relation to cholangiocarcinoma progression. In Asian Pacific journal of cancer prevention : APJCP, 15, 10187-92. doi:. https://pubmed.ncbi.nlm.nih.gov/25556446/
7. Matsuoka, Hiroshi, Katayama, Miyu, Ohishi, Ami, Shima, Akiho, Michihara, Akihiro. 2020. Orphan Nuclear Receptor RORα Regulates Enzymatic Metabolism of Cerebral 24S-Hydroxycholesterol through CYP39A1 Intronic Response Element Activation. In International journal of molecular sciences, 21, . doi:10.3390/ijms21093309. https://pubmed.ncbi.nlm.nih.gov/32392803/
8. Grabovec, I P, Smolskaya, S V, Baranovsky, A V, Usanov, S A, Strushkevich, N V. 2019. Ligand-binding properties and catalytic activity of the purified human 24-hydroxycholesterol 7α-hydroxylase, CYP39A1. In The Journal of steroid biochemistry and molecular biology, 193, 105416. doi:10.1016/j.jsbmb.2019.105416. https://pubmed.ncbi.nlm.nih.gov/31247323/
9. Ikeda, Hiromi, Ueda, Masamichi, Ikeda, Masataka, Kobayashi, Hiroshi, Honda, Yoshihito. . Oxysterol 7alpha-hydroxylase (CYP39A1) in the ciliary nonpigmented epithelium of bovine eye. In Laboratory investigation; a journal of technical methods and pathology, 83, 349-55. doi:. https://pubmed.ncbi.nlm.nih.gov/12649335/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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