Suclg2-flox Mouse
一般名
Suclg2-flox
製品ID
S-CKO-18019
背景情報
C57BL/6NCya
系統ID
CKOCMP-20917-Suclg2-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Suclg2-flox Mouse（カタログ番号S-CKO-18019）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Suclg2-flox
系統ID
CKOCMP-20917-Suclg2-B6N-VA
遺伝子名
製品ID
S-CKO-18019
遺伝子別名
G-SCS, GTPSCS, D6Wsu120e, SCS-betaG
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conditional knockout
染色体
Chr 6
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000204224
NCBIトランスクリプトID
NM_011507
ターゲット領域
Exon 3~4
有効領域の大きさ
~1.7 kb
遺伝子研究の概要
Suclg2, the succinyl-coenzyme A (CoA) synthetase GDP-forming subunit β, is involved in important metabolic processes. It participates in the tricarboxylic acid cycle by catalyzing the conversion of succinyl-CoA to succinate, generating GTP in the process. This function is crucial for mitochondrial energy production and maintaining normal cellular metabolism [3].
In lung adenocarcinoma, deletion of Suclg2 upregulates the succinylation level of mitochondrial proteins, inhibits key metabolic enzymes, and dampens mitochondrial function. Suclg2 itself is succinylated on Lys93, enhancing its protein stability, promoting cell proliferation and tumorigenesis [1].
In regulatory dendritic cells (diffDCs), Suclg2-interference impairs the tolerogenic function of diffDCs, enhances NF-κB signaling and expression of inflammatory genes, indicating its role in maintaining the immunoregulatory function of diffDCs [2].
In pheochromocytoma and paraganglioma, germline variants in Suclg2 lead to absence of its protein, decrease in SDHB subunit of SDH, faulty assembly of complex II, aberrant respiration, and elevated succinate accumulation [3].
In prostate cancer, ADT-induced upregulation of Suclg2 is associated with NE differentiation, and knockdown of Suclg2 suppresses NE differentiation and tumor growth [4].
In rheumatoid arthritis, SUCLG2-deficient T cells differentiate into pro-inflammatory effector cells, and preventing tubulin acetylation in these cells can inhibit synovitis [5].
In Alzheimer's disease, a single-nucleotide polymorphism in SUCLG2 is associated with CSF Aβ1-42 levels and rate of cognitive decline, and it is also included in the diagnostic panel for mild cognitive impairment [6,7].
In conclusion, Suclg2 plays essential roles in multiple biological processes and diseases. Its functions in mitochondrial metabolism, immune regulation, and tumorigenesis are revealed through various loss-of-function experiments. These findings contribute to understanding the mechanisms of diseases such as cancer, immune-related disorders, and neurodegenerative diseases, potentially providing new targets for treatment.
References：
1. Hu, Qifan, Xu, Jing, Wang, Lei, Han, Tianyu, Wang, Jian-Bin. 2023. SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303535. doi:10.1002/advs.202303535. https://pubmed.ncbi.nlm.nih.gov/37904651/
2. Zhang, Xiaomin, Liu, Juan, Cheng, Yujie, Liu, Shuxun, Cao, Xuetao. 2023. Metabolic enzyme Suclg2 maintains tolerogenicity of regulatory dendritic cells diffDCs by suppressing Lactb succinylation. In Journal of autoimmunity, 138, 103048. doi:10.1016/j.jaut.2023.103048. https://pubmed.ncbi.nlm.nih.gov/37216870/
3. Hadrava Vanova, Katerina, Pang, Ying, Krobova, Linda, Yang, Chunzhang, Pacak, Karel. . Germline SUCLG2 Variants in Patients With Pheochromocytoma and Paraganglioma. In Journal of the National Cancer Institute, 114, 130-138. doi:10.1093/jnci/djab158. https://pubmed.ncbi.nlm.nih.gov/34415331/
4. Lin, Shian-Ren, Wen, Yu-Ching, Yeh, Hsiu-Lien, Chen, Wei-Yu, Liu, Yen-Nien. 2020. EGFR-upregulated LIFR promotes SUCLG2-dependent castration resistance and neuroendocrine differentiation of prostate cancer. In Oncogene, 39, 6757-6775. doi:10.1038/s41388-020-01468-9. https://pubmed.ncbi.nlm.nih.gov/32963351/
5. Wu, Bowen, Qiu, Jingtao, Zhao, Tuantuan V, Goronzy, Jörg J, Weyand, Cornelia M. . Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. In Cell metabolism, 32, 967-980.e5. doi:10.1016/j.cmet.2020.10.025. https://pubmed.ncbi.nlm.nih.gov/33264602/
6. Ramirez, Alfredo, van der Flier, Wiesje M, Herold, Christine, Becker, Tim, Nöthen, Markus M. 2014. SUCLG2 identified as both a determinator of CSF Aβ1-42 levels and an attenuator of cognitive decline in Alzheimer's disease. In Human molecular genetics, 23, 6644-58. doi:10.1093/hmg/ddu372. https://pubmed.ncbi.nlm.nih.gov/25027320/
7. Wang, Yuye, Sun, Yu, Wang, Yu, Wang, Yi, Peng, Dantao. 2023. Identification of novel diagnostic panel for mild cognitive impairment and Alzheimer's disease: findings based on urine proteomics and machine learning. In Alzheimer's research & therapy, 15, 191. doi:10.1186/s13195-023-01324-4. https://pubmed.ncbi.nlm.nih.gov/37925455/
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精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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