Slc9a1-flox Mouse
一般名
Slc9a1-flox
製品ID
S-CKO-18194
背景情報
C57BL/6JCya
系統ID
CKOCMP-20544-Slc9a1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Slc9a1-flox Mouse（カタログ番号S-CKO-18194）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Slc9a1-flox
系統ID
CKOCMP-20544-Slc9a1-B6J-VB
遺伝子名
製品ID
S-CKO-18194
遺伝子別名
swe, Apnh, Nhe1, Mir5122, mir-5122
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 4
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000030669
NCBIトランスクリプトID
NM_016981
ターゲット領域
Exon 5
有効領域の大きさ
~1.0 kb
遺伝子研究の概要
Slc9a1, encoding the sodium/hydrogen exchanger isoform one (NHE1), is crucial for maintaining intracellular pH homeostasis by exchanging one intracellular proton for one extracellular sodium ion [3,4]. This process is involved in multiple biological pathways and is of great biological importance. Genetic models, such as knockout mouse models, have been valuable in studying its functions.
In conditional Slc9a1 knockout (cKO) mouse models, deletion of Slc9a1 in Cx3cr1+ cells led to expansion of a microglial subgroup with elevated transcription of white matter myelination genes. This subgroup also showed more acidic pHi and upregulated CREB signaling. Moreover, there was an enrichment of a corresponding oligodendrocyte subgroup in the white matter tissues, indicating stimulation of microglia-oligodendrocyte crosstalk for remyelination [1].
In mice, the Slc9a1 inhibitor decreased the transport efficiency of chitosan oligosaccharide (COS) both in vitro and in vivo, and overexpression of Slc9a1 in MODE-K cells increased COS transport, suggesting its role in COS transport across the intestinal mucosa [2].
Mice with a homozygous null mutation in Slc9a1 exhibited ataxia, recurrent seizures, and selective neuronal cell death, and in humans, mutations in SLC9A1 are associated with cerebellar ataxia, sensorineural hearing loss, and neuromuscular disorders [3,4].
In gliomas, higher SLC9A1 mRNA levels in higher-grade gliomas were correlated with worsened survival probabilities, and inhibition of NHE1 protein reduced glioma volume and invasion in mouse models [5]. Adipose mesenchymal stem cell-derived exosomal microRNA-1236 reduced breast cancer cells' resistance to cisplatin by suppressing SLC9A1 [6].
In conclusion, Slc9a1 plays essential roles in maintaining intracellular pH, microglia-oligodendrocyte crosstalk, intestinal transport, and is associated with various diseases including neurological disorders and cancers. The Slc9a1 KO/CKO mouse models have significantly contributed to understanding its functions in these disease-related biological processes.
References：
1. Song, Shanshan, Oft, Helena, Metwally, Shamseldin, Kohanbash, Gary, Sun, Dandan. 2024. Deletion of Slc9a1 in Cx3cr1+ cells stimulated microglial subcluster CREB1 signaling and microglia-oligodendrocyte crosstalk. In Journal of neuroinflammation, 21, 69. doi:10.1186/s12974-024-03065-z. https://pubmed.ncbi.nlm.nih.gov/38509618/
2. Wen, Jiaying, Chen, Shengwei, Bao, Minglong, Abd El-Aty, A M, Ju, Xianghong. 2022. Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice. In Carbohydrate polymers, 299, 120179. doi:10.1016/j.carbpol.2022.120179. https://pubmed.ncbi.nlm.nih.gov/36876794/
3. Iwama, Kazuhiro, Osaka, Hitoshi, Ikeda, Takahiro, Mizuguchi, Takeshi, Matsumoto, Naomichi. 2018. A novel SLC9A1 mutation causes cerebellar ataxia. In Journal of human genetics, 63, 1049-1054. doi:10.1038/s10038-018-0488-x. https://pubmed.ncbi.nlm.nih.gov/30018422/
4. Guissart, Claire, Li, Xiuju, Leheup, Bruno, Fliegel, Larry, Koenig, Michel. 2014. Mutation of SLC9A1, encoding the major Na⁺/H⁺ exchanger, causes ataxia-deafness Lichtenstein-Knorr syndrome. In Human molecular genetics, 24, 463-70. doi:10.1093/hmg/ddu461. https://pubmed.ncbi.nlm.nih.gov/25205112/
5. Guan, Xiudong, Luo, Lanxin, Begum, Gulnaz, Sun, Dandan, Jia, Wang. 2018. Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas. In Journal of experimental & clinical cancer research : CR, 37, 255. doi:10.1186/s13046-018-0923-z. https://pubmed.ncbi.nlm.nih.gov/30333031/
6. Jia, Zhongming, Zhu, Huamin, Sun, Hongguang, Jiang, Jingru, Wang, Xiaohong. 2020. Adipose Mesenchymal Stem Cell-Derived Exosomal microRNA-1236 Reduces Resistance of Breast Cancer Cells to Cisplatin by Suppressing SLC9A1 and the Wnt/β-Catenin Signaling. In Cancer management and research, 12, 8733-8744. doi:10.2147/CMAR.S270200. https://pubmed.ncbi.nlm.nih.gov/33061571/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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