Prkaa1-KO Mouse
一般名
Prkaa1-KO
製品ID
S-KO-00422
背景情報
C57BL/6JCya
系統ID
KOCMP-105787-Prkaa1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Prkaa1-KO Mouse（カタログ番号S-KO-00422）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Prkaa1-KO
系統ID
KOCMP-105787-Prkaa1-B6J-VA
遺伝子名
製品ID
S-KO-00422
遺伝子別名
AMPKalpha1, C130083N04Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 15
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000051186
NCBIトランスクリプトID
NM_001013367
ターゲット領域
Exon 3
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Prkaa1, also known as AMPKα1, is the α -subunit of 5 -AMP -activated protein kinase. This kinase is a major cellular sensor of energy and nutrients, playing a key role in regulating cellular energy metabolism through phosphorylation [1,2,7]. It is involved in multiple biological processes and associated with various pathways, such as autophagy, redox balance, and mitophagy, which are crucial for cell survival, development, and homeostasis [5,6,9].
In gene-knockout (KO) mouse models, endothelial-specific Prkaa1 knockout in HFD-fed mice alleviated HFD-induced metabolic syndromes, including decreased body weight, enhanced glucose clearance, and relieved adipose inflammation and hepatic steatosis. Mechanistically, PRKAA1 knockdown in cultured endothelial cells reduced glycolysis, fatty acid oxidation, and suppressed transcription of inflammatory molecules [2]. In Prkaa1-deficient mice, autophagy-dependent mitochondrial clearance during erythrocyte maturation was defective, leading to enhanced oxidative stress, shortened erythrocyte lifespan, and anemia [9].
In conclusion, Prkaa1 is essential for regulating cellular energy metabolism, autophagy, and redox balance. The Prkaa1 KO mouse models have revealed its significant role in metabolic syndrome and erythrocyte maturation. Additionally, studies on Prkaa1 genetic polymorphisms have shown its association with the risk of gastric and esophageal cancers, indicating its importance in cancer-related research [1,3,4,8].
References:
1. Chen, Yongyi, Chen, Siyu, Zhu, Jing, Yu, Qiong, Xu, Songxiao. 2023. PRKAA1 predicts prognosis and is associated with immune characteristics in gastric cancer. In Functional & integrative genomics, 23, 252. doi:10.1007/s10142-023-01176-z. https://pubmed.ncbi.nlm.nih.gov/37482545/
2. Yang, Qiuhua, Ma, Qian, Xu, Jiean, Belin de Chantemèle, Eric J, Huo, Yuqing. 2021. Endothelial AMPKα1/PRKAA1 exacerbates inflammation in HFD-fed mice. In British journal of pharmacology, 179, 1661-1678. doi:10.1111/bph.15742. https://pubmed.ncbi.nlm.nih.gov/34796475/
3. Jiang, You, Li, Wenbo, Lu, Jun, Zhao, Xin, Li, Liang. . Association between PRKAA1 rs13361707 T>C polymorphism and gastric cancer risk: Evidence based on a meta-analysis. In Medicine, 97, e0302. doi:10.1097/MD.0000000000010302. https://pubmed.ncbi.nlm.nih.gov/29620653/
4. Yu, Shuyong, Tu, Ruisha, Chen, Zhaowei, Zhang, Ronglin, Li, Yini. 2023. Association of PTGER4 and PRKAA1 genetic polymorphisms with gastric cancer. In BMC medical genomics, 16, 209. doi:10.1186/s12920-023-01645-1. https://pubmed.ncbi.nlm.nih.gov/37670284/
5. Zhang, Yangmei, Zhou, Xichang, Cheng, Xue, Chen, Kai, Li, Yang. 2022. PRKAA1, stabilized by FTO in an m6A-YTHDF2-dependent manner, promotes cell proliferation and glycolysis of gastric cancer by regulating the redox balance. In Neoplasma, 69, 1338-1348. doi:10.4149/neo_2022_220714N714. https://pubmed.ncbi.nlm.nih.gov/36305690/
6. Tang, Yanling, Zhang, Jingjing, Hu, Zeyu, Xing, Hengrui, Niu, Qiang. 2023. PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity. In Ecotoxicology and environmental safety, 255, 114772. doi:10.1016/j.ecoenv.2023.114772. https://pubmed.ncbi.nlm.nih.gov/36924562/
7. Zhang, Yangmei, Zhou, Xichang, Cheng, Long, Zhang, Youwei, Sun, Sanyuan. 2019. PRKAA1 Promotes Proliferation and Inhibits Apoptosis of Gastric Cancer Cells Through Activating JNK1 and Akt Pathways. In Oncology research, 28, 213-223. doi:10.3727/096504019X15668125347026. https://pubmed.ncbi.nlm.nih.gov/31558185/
8. Li, Cheng-Lin, Zhao, Jian-Qiang, Zang, Bao. 2020. PRKAA1 rs13361707 C/T polymorphism confers decreased susceptibility to esophageal cancer: A case-control study. In Journal of clinical laboratory analysis, 34, e23406. doi:10.1002/jcla.23406. https://pubmed.ncbi.nlm.nih.gov/32488984/
9. Zhu, Huaiping, Foretz, Marc, Xie, Zhonglin, Viollet, Benoit, Zou, Ming-Hui. 2014. PRKAA1/AMPKα1 is required for autophagy-dependent mitochondrial clearance during erythrocyte maturation. In Autophagy, 10, 1522-34. doi:10.4161/auto.29197. https://pubmed.ncbi.nlm.nih.gov/24988326/
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