Clic1-KO Mouse
一般名
Clic1-KO
製品ID
S-KO-00866
背景情報
C57BL/6NCya
系統ID
KOCMP-114584-Clic1-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Clic1-KO Mouse（カタログ番号S-KO-00866）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Clic1-KO
系統ID
KOCMP-114584-Clic1-B6N-VA
遺伝子名
製品ID
S-KO-00866
遺伝子別名
G6, Clcp
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 17
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000007257
NCBIトランスクリプトID
NM_033444
ターゲット領域
Exon 2~4
有効領域の大きさ
~0.7 kb
遺伝子研究の概要
CLIC1, or Chloride intracellular channel 1, can exist as a monomeric soluble or non-covalent dimeric protein capable of forming an ion channel. It plays diverse roles in cell cycle progression, cell volume regulation, and is involved in multiple cellular processes such as proliferation, invasion, migration, and angiogenesis [1]. It is associated with pathways like Wnt/β-catenin/TCF4, ROS/HIF1α, and Nrf2/HO-1, and is of great biological importance, especially in cancer biology and some inflammatory and metabolic diseases [2,6]. Genetic models are valuable for studying its functions.
In cancer, CLIC1 has been widely studied. In glioblastoma, it facilitates the G1/S phase transition and regulates glioma stem-like cells. Knockdown of CLIC1 in glioma cells led to apoptosis and attenuated cell motility [1,4]. In pancreatic cancer, elevated CLIC1, induced by matrix stiffness, promotes glycolytic metabolism and tumor proliferation [2]. In hepatocellular carcinoma, CLIC1 drives angiogenesis by modulating VEGFA, and knockdown of CLIC1 in Huh7 cells restrained xenograft tumor growth [3]. In esophageal cancer, KCTD4 binds to CLIC1, disrupts its dimerization, increases intracellular Ca2+ levels, and promotes metastasis, and a compound targeting the KCTD4-CLIC1 interaction suppresses cancer metastasis [7]. In oral squamous cell carcinoma, patients with nodal metastases had higher CLIC1 plasma concentration levels [5]. In addition, in obesity mouse models, Clic1 knockout mice ate less and had lower body weight, and pharmacological inhibition of Clic1 also reduced food intake and promoted weight loss [8].
In conclusion, CLIC1 is a multifunctional protein involved in various biological processes. Model-based research, especially through gene knockout in mouse models, has revealed its crucial role in cancer progression, angiogenesis, and obesity-related food intake regulation. These findings provide potential therapeutic targets for treating cancer and obesity.
References:
1. Randhawa, Kamaldeep, Jahani-Asl, Arezu. 2023. CLIC1 regulation of cancer stem cells in glioblastoma. In Current topics in membranes, 92, 99-123. doi:10.1016/bs.ctm.2023.09.004. https://pubmed.ncbi.nlm.nih.gov/38007271/
2. Zheng, Jia-Hao, Zhu, Yu-Heng, Yang, Jian, Sun, Yong-Wei, Liu, De-Jun. 2024. A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer. In Cell reports, 43, 114633. doi:10.1016/j.celrep.2024.114633. https://pubmed.ncbi.nlm.nih.gov/39154343/
3. Wei, Xuyong, Pan, Binhua, Yang, Mengfan, Lin, Hanchao, Xu, Xiao. . CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA. In Technology in cancer research & treatment, 21, 15330338221106820. doi:10.1177/15330338221106820. https://pubmed.ncbi.nlm.nih.gov/35722791/
4. Wang, Chengcheng, He, Zheng. 2023. Multi-omics analysis reveals CLIC1 as a therapeutic vulnerability of gliomas. In Frontiers in pharmacology, 14, 1279370. doi:10.3389/fphar.2023.1279370. https://pubmed.ncbi.nlm.nih.gov/38027011/
5. Wojtera, Bartosz Paweł, Sobecka, Agnieszka, Szewczyk, Mateusz, Suchorska, Wiktoria Maria, Golusiński, Wojciech. . CLIC1 plasma concentration is associated with lymph node metastases in oral squamous cell carcinoma. In Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 32, 341-347. doi:10.17219/acem/154621. https://pubmed.ncbi.nlm.nih.gov/36251793/
6. Lu, Dezhao, Le, Yifei, Ding, Jiali, Mao, Wei, Zhu, Ji. 2021. CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway. In Cell biochemistry and biophysics, 79, 239-252. doi:10.1007/s12013-020-00959-6. https://pubmed.ncbi.nlm.nih.gov/33432550/
7. Zheng, Cancan, Yu, Xiaomei, Xu, Taoyang, Liu, Jinbao, Xu, Wen Wen. 2023. KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer. In Acta pharmaceutica Sinica. B, 13, 4217-4233. doi:10.1016/j.apsb.2023.07.013. https://pubmed.ncbi.nlm.nih.gov/37799381/
8. Zapata, Rizaldy C, Zhang, Dinghong, Yoon, Dongmin, Petrascheck, Michael, Osborn, Olivia. 2023. Targeting Clic1 for the treatment of obesity: A novel therapeutic strategy to reduce food intake and body weight. In Molecular metabolism, 76, 101794. doi:10.1016/j.molmet.2023.101794. https://pubmed.ncbi.nlm.nih.gov/37604246/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。