Ctsk-KO Mouse
一般名
Ctsk-KO
製品ID
S-KO-01691
背景情報
C57BL/6JCya
系統ID
KOCMP-13038-Ctsk-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Ctsk-KO Mouse（カタログ番号S-KO-01691）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ctsk-KO
系統ID
KOCMP-13038-Ctsk-B6J-VB
遺伝子名
製品ID
S-KO-01691
遺伝子別名
catK, Ms10q, MMS10-Q
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000015664
NCBIトランスクリプトID
NM_007802
ターゲット領域
Exon 2~4
有効領域の大きさ
~2.1 kb
遺伝子研究の概要
Cathepsin K (Ctsk), a cysteine protease, is well-known for its strong activity in degrading the extracellular matrix. It is closely related to osteoclast-mediated bone destruction and plays a role in bone resorption [3]. Mutations in the Ctsk gene have been linked to pycnodysostosis, a rare autosomal recessive skeletal dysplasia [5,6,7,8,9].
In a mouse model where Trp53 and Rb1 were ablated in Ctsk-expressing cells (Ctsk-Cre;Trp53f/f/Rb1f/f mice), the animals spontaneously developed osteosarcoma with age, and loss of Trp53/Rb1 in these cells promoted the expression and nuclear translocation of YAP/TAZ. Verteporfin, an inhibitor of YAP/TAZ signaling, delayed osteosarcoma progression in these mice [2]. In another mouse FeCl3 model, Ctsk deficiency prevented stress-related thrombosis, as Ctsk seemed to contribute to thrombosis possibly via modulating vascular inflammation, oxidative production, and apoptosis [4]. In castration-resistant prostate cancer (CRPC), Ctsk was significantly up-regulated, and it regulated tumor growth and metastasis through the IL-17/CTSK/EMT axis and mediated M2 macrophage polarization [1].
In summary, Ctsk is crucial for extracellular matrix degradation and bone resorption. Gene-modified mouse models, such as Ctsk-Cre;Trp53f/f/Rb1f/f and Ctsk-deficient mice, have revealed its roles in osteosarcoma development, stress-related thrombosis, and CRPC, providing potential therapeutic targets for these diseases.
References:
1. Wu, Ning, Wang, YouZhi, Wang, KeKe, Liang, JiaMing, Jiang, Ning. 2022. Cathepsin K regulates the tumor growth and metastasis by IL-17/CTSK/EMT axis and mediates M2 macrophage polarization in castration-resistant prostate cancer. In Cell death & disease, 13, 813. doi:10.1038/s41419-022-05215-8. https://pubmed.ncbi.nlm.nih.gov/36138018/
2. Li, Yang, Yang, Shuting, Yang, Shuying. 2022. Verteporfin Inhibits the Progression of Spontaneous Osteosarcoma Caused by Trp53 and Rb1 Deficiency in Ctsk-Expressing Cells via Impeding Hippo Pathway. In Cells, 11, . doi:10.3390/cells11081361. https://pubmed.ncbi.nlm.nih.gov/35456040/
3. Gao, Li-Hong, Li, Shan-Shan, Yue, Hua, Zhang, Zhen-Lin. 2020. Associations of Serum Cathepsin K and Polymorphisms in CTSK Gene With Bone Mineral Density and Bone Metabolism Markers in Postmenopausal Chinese Women. In Frontiers in endocrinology, 11, 48. doi:10.3389/fendo.2020.00048. https://pubmed.ncbi.nlm.nih.gov/32117071/
4. Jin, Xueying, Yue, Xueling, Huang, Zhe, Kuzuya, Masafumi, Cheng, Xian Wu. 2024. Cathepsin K deficiency prevented stress-related thrombosis in a mouse FeCl3 model. In Cellular and molecular life sciences : CMLS, 81, 205. doi:10.1007/s00018-024-05240-0. https://pubmed.ncbi.nlm.nih.gov/38703204/
5. Markova, Tatiana Vladimirovna, Kenis, Vladimir, Melchenko, Evgeniy, Nagornova, Tatiana, Dadali, Elena Leonidovna. 2022. Clinical and genetic characterization of three Russian patients with pycnodysostosis due to pathogenic variants in the CTSK gene. In Molecular genetics & genomic medicine, 10, e1904. doi:10.1002/mgg3.1904. https://pubmed.ncbi.nlm.nih.gov/35315254/
6. Huang, Xianglan, Qi, Xuan, Li, Mei, Hu, Ying Ying, Xia, Weibo. 2015. A Mutation in CTSK Gene in an Autosomal Recessive Pycnodysostosis Family of Chinese Origin. In Calcified tissue international, 96, 373-8. doi:10.1007/s00223-015-9963-y. https://pubmed.ncbi.nlm.nih.gov/25725806/
7. Sait, Haseena, Srivastava, Priyanka, Gupta, Neerja, Pabbati, Jayalakshmi, Phadke, Shubha R. 2021. Phenotypic and genotypic spectrum of CTSK variants in a cohort of twenty-five Indian patients with pycnodysostosis. In European journal of medical genetics, 64, 104235. doi:10.1016/j.ejmg.2021.104235. https://pubmed.ncbi.nlm.nih.gov/33945887/
8. Xue, Y, Wang, L, Xia, D, Mao, T, Duan, X. 2015. Dental Abnormalities Caused by Novel Compound Heterozygous CTSK Mutations. In Journal of dental research, 94, 674-81. doi:10.1177/0022034515573964. https://pubmed.ncbi.nlm.nih.gov/25731711/
9. Naeem, Muhammad, Sheikh, Sabeen, Ahmad, Wasim. 2009. A mutation in CTSK gene in an autosomal recessive pycnodysostosis family of Pakistani origin. In BMC medical genetics, 10, 76. doi:10.1186/1471-2350-10-76. https://pubmed.ncbi.nlm.nih.gov/19674475/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。