Lacc1-KO Mouse
一般名
Lacc1-KO
製品ID
S-KO-04761
背景情報
C57BL/6JCya
系統ID
KOCMP-210808-Lacc1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Lacc1-KO Mouse（カタログ番号S-KO-04761）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Lacc1-KO
系統ID
KOCMP-210808-Lacc1-B6J-VA
遺伝子名
製品ID
S-KO-04761
遺伝子別名
9030625A04Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 14
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000062789
NCBIトランスクリプトID
NM_172488
ターゲット領域
Exon 4~6
有効領域の大きさ
~4.1 kb
遺伝子研究の概要
Lacc1, also known as C13orf31, is an enzyme highly expressed in inflammatory macrophages. It serves a central regulatory role in multiple inflammatory diseases such as inflammatory bowel diseases, arthritis, and microbial infections [1,2,3,4,5,6,7,8]. Biochemically, Lacc1 converts L-citrulline to L-ornithine (L-Orn) and isocyanic acid, bridging proinflammatory nitric oxide synthase (NOS2) and polyamine immunometabolism [1,2].
In Lacc1 knockout (KO) mouse models, several key findings have emerged. Lacc1 -/- mice had more severe colon lesions following oral administration of Citrobacter rodentium, and an accelerated onset and worse arthritis in collagen-induced and mannan-induced arthritis models. Serum and local TNF were increased, and the percentage of IL-17A-producing CD4+ T cells was elevated [6]. In the context of intestinal inflammation and bacterial clearance, Lacc1 -/- mice developed more severe T-cell transfer colitis, had an increased bacterial burden in intestinal lymphoid organs, and altered T-cell cytokine profiles [7]. Also, Lacc1 deficiency in mice aggravated dextran sodium sulfate (DSS)-induced inflammatory bowel disease, with significant changes in the intestinal flora [8]. In humans, LACC1 deficiency in macrophages is linked to juvenile idiopathic arthritis, associated with autophagy and metabolism defects [3,5,9].
In conclusion, Lacc1 plays a crucial role in macrophage immunometabolism, inflammation regulation, and bacterial clearance. The study of Lacc1 KO mouse models has significantly enhanced our understanding of its functions in inflammatory diseases like arthritis and inflammatory bowel disease, providing insights into potential therapeutic targets for these conditions.
References:
1. Wei, Zheng, Oh, Joonseok, Flavell, Richard A, Crawford, Jason M. 2022. LACC1 bridges NOS2 and polyamine metabolism in inflammatory macrophages. In Nature, 609, 348-353. doi:10.1038/s41586-022-05111-3. https://pubmed.ncbi.nlm.nih.gov/35978195/
2. Li, Yaling, Wu, Zhixiong, Tan, Xiaoli, Tang, Liang, Ouyang, Fan. 2023. LACC1: A critical involvement in macrophage immunometabolism. In Cell biology international, 47, 1488-1490. doi:10.1002/cbin.12063. https://pubmed.ncbi.nlm.nih.gov/37366569/
3. Omarjee, Ommar, Mathieu, Anne-Laure, Quiniou, Gaëlle, Walzer, Thierry, Belot, Alexandre. . LACC1 deficiency links juvenile arthritis with autophagy and metabolism in macrophages. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20201006. https://pubmed.ncbi.nlm.nih.gov/33606008/
4. Xiong, Yulong, Zhang, Zhenhao, Liu, Shangyu, Zhou, Likun, Yao, Yan. 2023. Lupeol alleviates autoimmune myocarditis by suppressing macrophage pyroptosis and polarization via PPARα/LACC1/NF-κB signaling pathway. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 123, 155193. doi:10.1016/j.phymed.2023.155193. https://pubmed.ncbi.nlm.nih.gov/37976692/
5. He, Tingyan, Wang, Linlin, Huang, Xiaomei, Weng, Ruohang, Yang, Jun. 2024. LACC1 deficiency leading to juvenile arthritis and anemia. In Clinical immunology (Orlando, Fla.), 265, 110290. doi:10.1016/j.clim.2024.110290. https://pubmed.ncbi.nlm.nih.gov/38944365/
6. Skon-Hegg, Cara, Zhang, Juan, Wu, Xiumin, Lee, Wyne P, Behrens, Timothy W. 2018. LACC1 Regulates TNF and IL-17 in Mouse Models of Arthritis and Inflammation. In Journal of immunology (Baltimore, Md. : 1950), 202, 183-193. doi:10.4049/jimmunol.1800636. https://pubmed.ncbi.nlm.nih.gov/30510070/
7. Kang, Jung-Woo, Yan, Jie, Ranjan, Kishu, Turner, Jerrold R, Abraham, Clara. 2020. Myeloid Cell Expression of LACC1 Is Required for Bacterial Clearance and Control of Intestinal Inflammation. In Gastroenterology, 159, 1051-1067. doi:10.1053/j.gastro.2020.07.024. https://pubmed.ncbi.nlm.nih.gov/32693188/
8. Xu, Zheng-Yuan, Wang, Jin-Chun. 2023. LACC1 regulates changes in the intestinal flora in a mouse model of inflammatory bowel disease. In BMC gastroenterology, 23, 358. doi:10.1186/s12876-023-02971-5. https://pubmed.ncbi.nlm.nih.gov/37848840/
9. Clarke, Joanna. . LACC1-associated JIA linked to autophagy defects in macrophages. In Nature reviews. Rheumatology, 17, 251. doi:10.1038/s41584-021-00607-0. https://pubmed.ncbi.nlm.nih.gov/33762710/
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