Mboat4-KO Mouse
一般名
Mboat4-KO
製品ID
S-KO-06691
背景情報
C57BL/6NCya
系統ID
KOCMP-234155-Mboat4-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Mboat4-KO Mouse（カタログ番号S-KO-06691）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mboat4-KO
系統ID
KOCMP-234155-Mboat4-B6N-VA
遺伝子名
製品ID
S-KO-06691
遺伝子別名
GOAT, Gm171
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 8
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000095345
NCBIトランスクリプトID
NM_001126314
ターゲット領域
Exon 2~3
有効領域の大きさ
~4.1 kb
遺伝子研究の概要
Mboat4, also known as ghrelin O-acyl transferase (GOAT), is a crucial enzyme. It catalyzes the post-translational octanoylation of Ser-3 residue of ghrelin, a 28-residue peptide hormone produced by stomach P/D1 cells. This modification is essential for ghrelin to bind to its receptor in target tissues. Ghrelin is involved in multiple physiological processes such as regulating food intake, growth hormone secretion from the pituitary, and inhibiting insulin secretion from the pancreas, highlighting the biological importance of Mboat4 in these associated pathways [1,6,7,8,9].
Some research indicates Mboat4 may be involved in lipid metabolism as gene-educational attainment interactions identified it as part of novel lipid loci, suggesting its role in adipose biology [2]. In addition, in Japanese patients with schizophrenia, the mRNA expression of Mboat4 was significantly increased, potentially reflecting the mechanisms of the disease [3]. Also, in silico studies identified pathogenic SNPs in Mboat4, suggesting it could be an anti-obesity target as it catalyzes ghrelin acylation which is related to prominent ghrelin activity [4]. In Alzheimer's disease patients, the methylation rate of Mboat4 CpG 2 was lower and its mRNA expression was higher, which may be a neuroprotective biomarker [5].
In conclusion, Mboat4 is essential for ghrelin activation and thus for various physiological functions related to metabolism and hormone regulation. Studies on Mboat4 in different disease conditions such as schizophrenia, Alzheimer's disease, and obesity-related research contribute to understanding its role in these pathologies, potentially providing new directions for therapeutic interventions.
References:
1. Murzinski, Emily S, Saha, Ishika, Ding, Hui, Tontonoz, Peter, Harran, Patrick G. 2021. In Search of Small Molecules That Selectively Inhibit MBOAT4. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26247599. https://pubmed.ncbi.nlm.nih.gov/34946685/
2. de las Fuentes, Lisa, Schwander, Karen L, Brown, Michael R, Rotter, Jerome I, Fornage, Myriam. 2023. Gene-educational attainment interactions in a multi-population genome-wide meta-analysis identify novel lipid loci. In Frontiers in genetics, 14, 1235337. doi:10.3389/fgene.2023.1235337. https://pubmed.ncbi.nlm.nih.gov/38028628/
3. Nakata, Shunsuke, Yoshino, Yuta, Okita, Mitsuo, Iga, Jun-Ichi, Ueno, Shu-Ichi. 2018. Differential expression of the ghrelin-related mRNAs GHS-R1a, GHS-R1b, and MBOAT4 in Japanese patients with schizophrenia. In Psychiatry research, 272, 334-339. doi:10.1016/j.psychres.2018.12.135. https://pubmed.ncbi.nlm.nih.gov/30597386/
4. Azmi, Muhammad Bilal, Sehgal, Sheikh Arslan, Asif, Uzma, Ahmed, Syed Danish Haseen, Qureshi, Shamim Akhtar. 2023. Genetic insights into obesity: in silico identification of pathogenic SNPs in MBOAT4 gene and their structural molecular dynamics consequences. In Journal of biomolecular structure & dynamics, 42, 13074-13090. doi:10.1080/07391102.2023.2274970. https://pubmed.ncbi.nlm.nih.gov/37921712/
5. Yoshino, Yuta, Funahashi, Yu, Nakata, Shunsuke, Iga, Jun-Ichi, Ueno, Shu-Ichi. 2018. Ghrelin cascade changes in the peripheral blood of Japanese patients with Alzheimer's disease. In Journal of psychiatric research, 107, 79-85. doi:10.1016/j.jpsychires.2018.10.011. https://pubmed.ncbi.nlm.nih.gov/30366284/
6. Sato, Takahiro, Ida, Takanori, Nakamura, Yuki, Kangawa, Kenji, Kojima, Masayasu. 2013. Physiological roles of ghrelin on obesity. In Obesity research & clinical practice, 8, e405-13. doi:10.1016/j.orcp.2013.10.002. https://pubmed.ncbi.nlm.nih.gov/25263830/
7. Taylor, Martin S, Hwang, Yousang, Hsiao, Po-Yuan, Boeke, Jef D, Cole, Philip A. . Ghrelin O-acyltransferase assays and inhibition. In Methods in enzymology, 514, 205-28. doi:10.1016/B978-0-12-381272-8.00013-1. https://pubmed.ncbi.nlm.nih.gov/22975055/
8. Abizaid, Alfonso, Hougland, James L. 2019. Ghrelin Signaling: GOAT and GHS-R1a Take a LEAP in Complexity. In Trends in endocrinology and metabolism: TEM, 31, 107-117. doi:10.1016/j.tem.2019.09.006. https://pubmed.ncbi.nlm.nih.gov/31636018/
9. Delhanty, P J D, van der Lely, A J. 2011. Ghrelin and glucose homeostasis. In Peptides, 32, 2309-18. doi:10.1016/j.peptides.2011.03.001. https://pubmed.ncbi.nlm.nih.gov/21396419/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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