Mindy1-KO Mouse
一般名
Mindy1-KO
製品ID
S-KO-14550
背景情報
C57BL/6NCya
系統ID
KOCMP-75007-Mindy1-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Mindy1-KO Mouse（カタログ番号S-KO-14550）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mindy1-KO
系統ID
KOCMP-75007-Mindy1-B6N-VA
遺伝子名
製品ID
S-KO-14550
遺伝子別名
ni, cI-40, Fam63a, mKIAA1390, 1810005H09Rik, 4930504E06Rik
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000107187
NCBIトランスクリプトID
NM_199475
ターゲット領域
Exon 2~5
有効領域の大きさ
~4.6 kb
遺伝子研究の概要
MINDY1, a member of the motif interacting with ubiquitin-containing novel DUB family, is a deubiquitinating enzyme. It has a key function in regulating protein stability by cleaving K48-linked polyubiquitin chains, thus affecting various biological processes and disease-related pathways [5].
In cancer research, MINDY1 has been found to play significant roles. In bladder cancer, MINDY1 depletion decreased cell proliferation by reducing YAP protein level and YAP/TEAD target genes expression, suggesting it promotes bladder cancer progression by stabilizing YAP [1]. In breast cancer, MINDY1 interacts with estrogen receptor α (ERα), mediates its deubiquitination, and increases its stability. MINDY1 depletion led to growth inhibition and cell cycle arrest of ERα-positive breast cancer cells [2]. In liver cancer, knocking out MINDY1 reduced the expression of stem markers, self-renewal ability of cells, and growth of transplanted tumors, and its high expression is an independent risk factor for poor prognosis [6]. Also, in breast and liver cancer, MINDY1 facilitates immune escape by maintaining the stability of immune checkpoint protein PD-L1 [7,8]. In muscle-related research, MINDY1 knockdown enhances insulin-stimulated glucose uptake in rat myotubes, linking it to insulin action in skeletal muscle [3]. In embryonic stem cells, high polyamine levels promote ESC self-renewal, in part, through maintaining high MINDY1 levels [4].
In conclusion, MINDY1 is a crucial deubiquitinating enzyme. Its function in stabilizing key proteins has a significant impact on cancer development, including bladder, breast, and liver cancer, as well as in processes like insulin action and embryonic stem cell self-renewal. Research using gene-knockout models in these areas has been instrumental in revealing these functions, providing potential therapeutic targets for related diseases.
References:
1. Luo, Yongwen, Zhou, Jun, Tang, Jianing, Liu, Tongzu, Liu, Tao. 2021. MINDY1 promotes bladder cancer progression by stabilizing YAP. In Cancer cell international, 21, 395. doi:10.1186/s12935-021-02095-4. https://pubmed.ncbi.nlm.nih.gov/34315490/
2. Tang, Jianing, Luo, Yongwen, Long, Guo, Zhou, Ledu. 2021. MINDY1 promotes breast cancer cell proliferation by stabilizing estrogen receptor α. In Cell death & disease, 12, 937. doi:10.1038/s41419-021-04244-z. https://pubmed.ncbi.nlm.nih.gov/34645792/
3. Needham, Elise J, Hingst, Janne R, Onslev, Johan D, James, David E, Wojtaszewski, Jørgen F P. 2024. Personalized phosphoproteomics of skeletal muscle insulin resistance and exercise links MINDY1 to insulin action. In Cell metabolism, 36, 2542-2559.e6. doi:10.1016/j.cmet.2024.10.020. https://pubmed.ncbi.nlm.nih.gov/39577414/
4. James, Christina, Zhao, Tian Yun, Rahim, Anisa, Dunn, Norris Ray, Vardy, Leah A. 2018. MINDY1 Is a Downstream Target of the Polyamines and Promotes Embryonic Stem Cell Self-Renewal. In Stem cells (Dayton, Ohio), 36, 1170-1178. doi:10.1002/stem.2830. https://pubmed.ncbi.nlm.nih.gov/29644784/
5. Abdul Rehman, Syed Arif, Armstrong, Lee A, Lange, Sven M, Svergun, Dmitri I, Kulathu, Yogesh. 2021. Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. In Molecular cell, 81, 4176-4190.e6. doi:10.1016/j.molcel.2021.08.024. https://pubmed.ncbi.nlm.nih.gov/34529927/
6. Xia, B L, Liu, K W, Huang, H X, Wang, B, Gao, J. . [Deubiquitinating enzyme MINDY1 is an independent risk factor for the maintenance of stemness and poor prognosis in liver cancer cells]. In Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, 31, 518-523. doi:10.3760/cma.j.cn501113-20230130-00028. https://pubmed.ncbi.nlm.nih.gov/37365029/
7. Ren, Liang, Wang, Li, Cao, Zhewei, Yang, Yang, Liu, Ya. 2024. Deubiquitinating Enzyme MINDY1 Facilitates Immune Escape in Breast Cancer by Maintaining the Stability of Immune Checkpoint Protein PD-L1. In The Tohoku journal of experimental medicine, , . doi:10.1620/tjem.2024.J111. https://pubmed.ncbi.nlm.nih.gov/39443136/
8. Song, Xingchao, Li, Wenjin, Tian, Chunyan, Yang, Weibin, Zhou, Jiahua. 2024. Study on the mechanism of liver cancer immune escape mediated by MINDY1 through regulation of PD-L1 ubiquitination level. In Biomolecules & biomedicine, 25, 144-154. doi:10.17305/bb.2024.10962. https://pubmed.ncbi.nlm.nih.gov/39217442/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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