Acot2-KO Mouse
一般名
Acot2-KO
製品ID
S-KO-16977
背景情報
C57BL/6JCya
系統ID
KOCMP-171210-Acot2-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Acot2-KO Mouse（カタログ番号S-KO-16977）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Acot2-KO
系統ID
KOCMP-171210-Acot2-B6J-VB
遺伝子名
製品ID
S-KO-16977
遺伝子別名
Mte1, MTE-I
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 12
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000021649
NCBIトランスクリプトID
NM_134188
ターゲット領域
Exon 2
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Acot2, or acyl-CoA thioesterase 2, hydrolyzes acyl-coenzyme A (CoA) into free fatty acids and CoA, thus playing a significant role in lipid metabolism pathways, such as fatty acid elongation and biosynthesis of unsaturated fatty acids [1,2,3,4,5,6,7,8]. It is expressed in highly oxidative tissues, including liver and skeletal muscle, and may contribute to maintaining the balance of free fatty acids and acyl CoA at the cellular level [2,3,4,6]. Genetic models could potentially be used to further study its functions.
In acute myeloid leukemia (AML), high expression of ACOT2 is associated with poor overall survival and abnormal lipid metabolism, making it a potential therapeutic target [1]. In Chinese Red Steppe cattle, overexpression of Acot2 promotes preadipocyte differentiation and lipid droplet accumulation, while its down-regulation inhibits these processes, suggesting it is a positive regulator of adipocyte differentiation [2]. In mice, adenoviral Acot2 overexpression in the liver increases fatty acid oxidation during the daytime, leading to higher serum ketones and less hepatic steatosis during overnight fasting [3]. In murine skeletal muscle, Acot2 deletion results in acyl-CoA build-up under certain conditions and affects the preference for glucose or fatty acid oxidation, as well as glucose homeostasis in high-fat-fed mice [4].
In conclusion, Acot2 is crucial for lipid metabolism, influencing processes like adipocyte differentiation, fatty acid oxidation, and glucose homeostasis. The use of genetic models, such as overexpression or deletion in mouse models, has provided insights into its role in diseases like AML and metabolic conditions related to lipid metabolism [1,2,3,4].
References:
1. Yin, Xuewei, Lyu, Chunyi, Li, Zonghong, Guo, Dadong, Xu, Ruirong. 2022. High Expression of ACOT2 Predicts Worse Overall Survival and Abnormal Lipid Metabolism: A Potential Target for Acute Myeloid Leukemia. In Journal of healthcare engineering, 2022, 2669114. doi:10.1155/2022/2669114. https://pubmed.ncbi.nlm.nih.gov/36193167/
2. Liu, Lixiang, Wu, Jian, Gao, Yi, Cao, Yang, Zhang, Guoliang. . The effect of Acot2 overexpression or downregulation on the preadipocyte differentiation in Chinese Red Steppe cattle. In Adipocyte, 9, 279-289. doi:10.1080/21623945.2020.1776553. https://pubmed.ncbi.nlm.nih.gov/32579860/
3. Moffat, Cynthia, Bhatia, Lavesh, Nguyen, Teresa, Claypool, Steven M, Seifert, Erin L. 2014. Acyl-CoA thioesterase-2 facilitates mitochondrial fatty acid oxidation in the liver. In Journal of lipid research, 55, 2458-70. doi:10.1194/jlr.M046961. https://pubmed.ncbi.nlm.nih.gov/25114170/
4. Bekeova, Carmen, Han, Ji In, Xu, Heli, Snyder, Nathaniel W, Seifert, Erin L. 2023. Acyl-CoA thioesterase-2 facilitates β-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.06.27.546724. https://pubmed.ncbi.nlm.nih.gov/37425757/
5. Momose, Atsushi, Fujita, Mariko, Ohtomo, Takayuki, Morikawa, Masako, Yamada, Junji. 2010. Regulated expression of acyl-CoA thioesterases in the differentiation of cultured rat brown adipocytes. In Biochemical and biophysical research communications, 404, 74-8. doi:10.1016/j.bbrc.2010.11.066. https://pubmed.ncbi.nlm.nih.gov/21094633/
6. Bekeova, Carmen, Anderson-Pullinger, Lauren, Boye, Kevin, Herrmann, Johannes M, Seifert, Erin L. 2019. Multiple mitochondrial thioesterases have distinct tissue and substrate specificity and CoA regulation, suggesting unique functional roles. In The Journal of biological chemistry, 294, 19034-19047. doi:10.1074/jbc.RA119.010901. https://pubmed.ncbi.nlm.nih.gov/31676684/
7. Del Duca, Ester, Dahabreh, Dante, Kim, Madeline, Agache, Ioana, Guttman-Yassky, Emma. 2024. Transcriptomic evaluation of skin tape-strips in children with allergic asthma uncovers epidermal barrier dysfunction and asthma-associated biomarkers abnormalities. In Allergy, 79, 1516-1530. doi:10.1111/all.16060. https://pubmed.ncbi.nlm.nih.gov/38375886/
8. Sun, Yuchen, Sun, Bo, Han, Xuesong, Shan, Anshan, Ma, Qingquan. . Leucine Supplementation Ameliorates Early-Life Programming of Obesity in Rats. In Diabetes, 72, 1409-1423. doi:10.2337/db22-0862. https://pubmed.ncbi.nlm.nih.gov/37196349/
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