Rcor1-KO Mouse
一般名
Rcor1-KO
製品ID
S-KO-17479
背景情報
C57BL/6JCya
系統ID
KOCMP-217864-Rcor1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Rcor1-KO Mouse（カタログ番号S-KO-17479）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Rcor1-KO
系統ID
KOCMP-217864-Rcor1-B6J-VA
遺伝子名
製品ID
S-KO-17479
遺伝子別名
Rocr1, D12Wsu95e, mKIAA0071, 5730409O11, 6720480E22Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 12
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000084968
NCBIトランスクリプトID
NM_198023
ターゲット領域
Exon 4
有効領域の大きさ
~1.1 kb
遺伝子研究の概要
RCOR1, also known as REST Corepressor 1, is a transcription repressor that recruits and positions LSD1 and HDAC1/2 on chromatin to modify histone methylation and acetylation. It is involved in multiple biological processes such as transcription regulation, cell differentiation, and development. It also plays a role in various signaling pathways, and its dysregulation is associated with diseases [1,2,3,4,5,6,7,8]. Mouse models have been crucial in understanding its functions in vivo [5,7].
In murine studies, Rcor1 -deficient mice are profoundly anemic and die in late gestation. Definitive erythroid cells from these mutant mice arrest at the transition from proerythroblast to basophilic erythroblast, and the mutant proerythroblasts aberrantly express genes of the myeloid lineage as well as HSC-related genes. Blocking the CSF2-dependent phospho-Stat5 hypersensitivity pathway can partially reduce myeloid colony formation by Rcor1-deficient erythroid progenitors [5]. Conditional knockout of Rcor1 in adult mice leads to severe anemia due to a block in committed erythroid progenitor maturation, reduced B-cells with increased apoptosis (non-cell autonomous requirement for B-cell survival), absence of mature neutrophils, and an increase in monocytic cells. Rcor1-deficient monocytes show enhanced self-renewal and overexpress genes associated with HSC/progenitor cell expansion [7]. In a DLBCL cohort, novel deletions in RCOR1 are associated with unfavorable progression-free survival, and an RCOR1 loss-associated gene signature can identify a subgroup of patients with unfavorable overall survival [8].
In conclusion, RCOR1 is essential for normal myeloerythroid lineage differentiation, and its deficiency leads to hematopoietic disorders. In addition, its dysregulation is associated with DLBCL prognosis. Mouse models, especially KO and CKO models, have been instrumental in revealing these functions and disease associations, providing insights into the biological processes and potential therapeutic targets related to RCOR1.
References：
1. Rivera, Carlos, Lee, Hun-Goo, Lappala, Anna, Lee, Jeannie T, Andrés, María Estela. 2022. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. In Nature communications, 13, 1550. doi:10.1038/s41467-022-29261-0. https://pubmed.ncbi.nlm.nih.gov/35322029/
2. Primrose, Julia G B, Jain, Lekha, Alhilali, Mariam, Dalbeth, Nicola, Poulsen, Raewyn C. 2023. REST, RCOR1 and RCOR2 expression is reduced in osteoarthritic chondrocytes and contributes to increasing MMP13 and ADAMTS5 expression through upregulating HES1. In Cellular signalling, 109, 110800. doi:10.1016/j.cellsig.2023.110800. https://pubmed.ncbi.nlm.nih.gov/37442513/
3. Liu, Chenli, Dong, Zhong, Li, Maozhang, Bai, Guangwei, Zhao, Zhixiang. 2024. RCOR1 is targeted by miR-23b-3p to modulate growth, colony formation, migration, and invasion of prostate cancer cells. In International journal of clinical and experimental pathology, 17, 29-38. doi:. https://pubmed.ncbi.nlm.nih.gov/38455506/
4. Xu, Hai, Yu, Xuetao, Xie, Rong, Wang, Yangyang, Li, Chunli. 2023. RCOR1 improves neurobehaviors and neuron injury in rat cerebral palsy by Endothelin-1 targeting-induced Akt/GSK-3β pathway upregulation. In Brain & development, 46, 93-102. doi:10.1016/j.braindev.2023.11.001. https://pubmed.ncbi.nlm.nih.gov/37978036/
5. Yao, Huilan, Goldman, Devorah C, Nechiporuk, Tamilla, Fleming, William H, Mandel, Gail. 2014. Corepressor Rcor1 is essential for murine erythropoiesis. In Blood, 123, 3175-84. doi:10.1182/blood-2013-11-538678. https://pubmed.ncbi.nlm.nih.gov/24652990/
6. Xiang, Zhao, Zhou, Shijie, Liang, Shuang, Zhang, Gang, Tan, Yinghui. 2020. RCOR1 directly binds to MED28 and weakens its inducing effect on cancer stem cell-like activity of oral cavity squamous cell carcinoma cells. In Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 49, 741-750. doi:10.1111/jop.13022. https://pubmed.ncbi.nlm.nih.gov/32306431/
7. Yao, Huilan, Goldman, Devorah C, Fan, Guang, Mandel, Gail, Fleming, William H. 2015. The Corepressor Rcor1 Is Essential for Normal Myeloerythroid Lineage Differentiation. In Stem cells (Dayton, Ohio), 33, 3304-14. doi:10.1002/stem.2086. https://pubmed.ncbi.nlm.nih.gov/26119982/
8. Chan, Fong Chun, Telenius, Adele, Healy, Shannon, Shah, Sohrab P, Steidl, Christian. 2014. An RCOR1 loss-associated gene expression signature identifies a prognostically significant DLBCL subgroup. In Blood, 125, 959-66. doi:10.1182/blood-2013-06-507152. https://pubmed.ncbi.nlm.nih.gov/25395426/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。