Folr2-KO Mouse
一般名
Folr2-KO
製品ID
S-KO-18565
背景情報
C57BL/6JCya
系統ID
KOCMP-14276-Folr2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Folr2-KO Mouse（カタログ番号S-KO-18565）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Folr2-KO
系統ID
KOCMP-14276-Folr2-B6J-VA
遺伝子名
製品ID
S-KO-18565
遺伝子別名
FBP2, FR-P3, Folbp2, FR-beta, Folbp-2
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 7
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000210598
NCBIトランスクリプトID
NM_001303239
ターゲット領域
Exon 4~6
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Folr2, also known as Folate Receptor 2, is a protein-coding gene. Folate receptors are important in folate transport, and Folr2 may be involved in processes related to one-carbon metabolism. Folr2-expressing cells, especially macrophages, play crucial roles in immune-related biological processes [5].
In cancer research, a discrete population of FOLR2+ tissue-resident macrophages has been identified in healthy mammary gland and breast cancer primary tumors. These macrophages localize in perivascular areas of the tumor stroma, interact with CD8+ T cells, and efficiently prime effector CD8+ T cells ex vivo. The density of FOLR2+ macrophages in breast tumors positively correlates with better patient survival [1]. In hepatocellular carcinoma, fetal-like (FOLR2) tumor-associated macrophages emerge as a result of onco-fetal reprogramming of the tumor microenvironment [2]. In chronic kidney disease, inflammatory fibroblasts promote the switch of macrophages into FOLR2+ macrophages, and this interaction is involved in kidney fibrosis [3]. In colon cancer, a subset of FOLR2+ macrophages is embedded in plasma cell niches [4]. In gastric cancer, FOLR2+ macrophages possess antitumor immune potential, and their proportion gradually decreases from complete intestinal metaplasia to incomplete intestinal metaplasia and early gastric cancer stages [6]. In lung adenocarcinoma, FOLR2-expressing tumor-associated macrophages may contribute to the formation of an immunosuppressive microenvironment through a specific cell-to-cell trajectory [7]. A population of TIM4+FOLR2+ macrophages localized in tertiary lymphoid structures correlates to an active immune infiltrate across several cancer types, with different phenotypes and prognostic associations [8].
In conclusion, Folr2, especially through its expression in macrophages, is involved in multiple disease-related immune processes, including cancer and chronic kidney disease. The study of Folr2-related macrophage populations in these disease conditions helps to understand the underlying immune-mediated mechanisms, potentially providing new targets for therapeutic interventions.
References：
1. Nalio Ramos, Rodrigo, Missolo-Koussou, Yoann, Gerber-Ferder, Yohan, Piaggio, Eliane, Helft, Julie. 2022. Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer. In Cell, 185, 1189-1207.e25. doi:10.1016/j.cell.2022.02.021. https://pubmed.ncbi.nlm.nih.gov/35325594/
2. Sharma, Ankur, Seow, Justine Jia Wen, Dutertre, Charles-Antoine, Ginhoux, Florent, DasGupta, Ramanuj. 2020. Onco-fetal Reprogramming of Endothelial Cells Drives Immunosuppressive Macrophages in Hepatocellular Carcinoma. In Cell, 183, 377-394.e21. doi:10.1016/j.cell.2020.08.040. https://pubmed.ncbi.nlm.nih.gov/32976798/
3. Cohen, Camille, Mhaidly, Rana, Croizer, Hugo, Ju, Wenjun, Mechta-Grigoriou, Fatima. 2024. WNT-dependent interaction between inflammatory fibroblasts and FOLR2+ macrophages promotes fibrosis in chronic kidney disease. In Nature communications, 15, 743. doi:10.1038/s41467-024-44886-z. https://pubmed.ncbi.nlm.nih.gov/38272907/
4. Matusiak, Magdalena, Hickey, John W, van IJzendoorn, David G P, West, Robert B, van de Rijn, Matt. . Spatially Segregated Macrophage Populations Predict Distinct Outcomes in Colon Cancer. In Cancer discovery, 14, 1418-1439. doi:10.1158/2159-8290.CD-23-1300. https://pubmed.ncbi.nlm.nih.gov/38552005/
5. Nawaz, Fathima Zahra, Kipreos, Edward T. 2022. Emerging roles for folate receptor FOLR1 in signaling and cancer. In Trends in endocrinology and metabolism: TEM, 33, 159-174. doi:10.1016/j.tem.2021.12.003. https://pubmed.ncbi.nlm.nih.gov/35094917/
6. He, Yuxin, Wang, Jiayu, Deng, Zilin, Shi, Tongguo, Chen, Weichang. 2024. FOLR2+ macrophage depletion from intestinal metaplasia to early gastric cancer: single-cell sequencing insight into gastric cancer progression. In Journal of experimental & clinical cancer research : CR, 43, 326. doi:10.1186/s13046-024-03245-y. https://pubmed.ncbi.nlm.nih.gov/39702278/
7. Xiang, Chan, Zhang, Min, Shang, Zhanxian, Yu, Yang, Han, Yuchen. 2023. Single-cell profiling reveals the trajectory of FOLR2-expressing tumor-associated macrophages to regulatory T cells in the progression of lung adenocarcinoma. In Cell death & disease, 14, 493. doi:10.1038/s41419-023-06021-6. https://pubmed.ncbi.nlm.nih.gov/37532692/
8. Bugatti, Mattia, Bergamini, Marco, Missale, Francesco, Benvenuti, Federica, Vermi, William. . A Population of TIM4+FOLR2+ Macrophages Localized in Tertiary Lymphoid Structures Correlates to an Active Immune Infiltrate Across Several Cancer Types. In Cancer immunology research, 10, 1340-1353. doi:10.1158/2326-6066.CIR-22-0271. https://pubmed.ncbi.nlm.nih.gov/36122412/
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