Mir125b-1-KO Mouse
一般名
Mir125b-1-KO
製品ID
S-KO-18879
背景情報
C57BL/6JCya
系統ID
KOCMP-387236-Mir125b-1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Mir125b-1-KO Mouse（カタログ番号S-KO-18879）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mir125b-1-KO
系統ID
KOCMP-387236-Mir125b-1-B6J-VA
遺伝子名
製品ID
S-KO-18879
遺伝子別名
Mirn125b, Mirn125b-1, mir-125b-1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 9
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000175613
NCBIトランスクリプトID
NR_029822
ターゲット領域
Exon 1
有効領域の大きさ
~0.1 kb
遺伝子研究の概要
Mir125b-1 is a microRNA-encoding gene. MicroRNAs are crucial regulators in various biological processes, often involved in post-transcriptional regulation of gene expression by binding to target mRNAs [1-8].
In human brain, Mir125b-1 is not imprinted, which is different from its isoform Mir125b-2. In mouse brain, it is highly expressed but down-regulated during development, with preferential expression in the olfactory bulb, thalamus, and hypothalamus, and is enriched in GABAergic neurons, especially somatostatin-expressing ones [1].
In metastatic clear cell renal cell carcinoma, high methylation levels of Mir125b-1 are correlated with late stages, large tumor size, metastasis, and loss of differentiation [2]. In breast cancer, increased methylation of Mir125b-1 in tumors compared to normal tissues was observed, and it was part of an optimized marker system for early diagnosis. Detection of its methylation was sufficient to classify samples as breast cancer [3].
In melanogenesis, reduction of miR-125b expression in pigmented cells was due to hypermethylation of the Mir125b-1 promoter [4]. In non-small cell lung cancer, a significantly high level of Mir125b-1 methylation was found in adenocarcinoma and squamous cell lung cancer compared to adjacent normal tissue [5].
In kidney cancer, increased methylation of Mir125b-1 was associated with distant metastases, and a combined panel including this gene could estimate metastasis probability with high accuracy [6]. In ovarian carcinoma, Mir125b-1 was hypermethylated at early stages, and in breast cancer, its hypermethylation was associated with lymph node metastasis and a system for predicting metastasis was developed based on it [7,8].
In conclusion, Mir125b-1 is involved in multiple biological and disease-related processes. Its non-imprinting in the human brain and specific spatiotemporal expression in the mouse brain contribute to understanding brain development. In various cancers such as renal, breast, lung, and ovarian cancers, changes in its methylation status are associated with tumor progression, metastasis, and can be used as potential biomarkers, highlighting the importance of studying Mir125b-1 in disease diagnosis and prognosis.
References：
1. Hou, Kuan-Chu, Tsai, Meng-Han, Akbarian, Schahram, Huang, Hsien-Sung. 2023. Mir125b-1 is Not Imprinted in Human Brain and Shows Developmental Expression Changes in Mouse Brain. In Neuroscience, 529, 99-106. doi:10.1016/j.neuroscience.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37598835/
2. Ivanova, N A, Burdennyi, A M, Lukina, S S, Braga, E A, Kushlinskii, N E. 2023. The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma. In Bulletin of experimental biology and medicine, 175, 249-253. doi:10.1007/s10517-023-05844-9. https://pubmed.ncbi.nlm.nih.gov/37466853/
3. Burdennyy, A M, Filippova, E A, Khodyrev, D S, Loginov, V I, Braga, E A. 2021. Optimized Marker System for Early Diagnosis of Breast Cancer. In Bulletin of experimental biology and medicine, 172, 57-62. doi:10.1007/s10517-021-05331-z. https://pubmed.ncbi.nlm.nih.gov/34791555/
4. Kim, Kyu-Han, Bin, Bum-Ho, Kim, Juewon, Cho, Eun-Gyung, Lee, Tae Ryong. 2013. Novel inhibitory function of miR-125b in melanogenesis. In Pigment cell & melanoma research, 27, 140-4. doi:10.1111/pcmr.12179. https://pubmed.ncbi.nlm.nih.gov/24118912/
5. Gubenko, M S, Loginov, V I, Burdennyy, A M, Kazubskaya, T P, Pertsov, S S. 2023. Changes in the Level of Methylation of a Group of microRNA Genes as a Factor in the Development and Progression of Non-Small Cell Lung Cancer. In Bulletin of experimental biology and medicine, 174, 254-258. doi:10.1007/s10517-023-05684-7. https://pubmed.ncbi.nlm.nih.gov/36598670/
6. Apanovich, Natalya, Matveev, Alexey, Ivanova, Natalia, Braga, Eleonora, Alimov, Andrei. 2023. Prediction of Distant Metastases in Patients with Kidney Cancer Based on Gene Expression and Methylation Analysis. In Diagnostics (Basel, Switzerland), 13, . doi:10.3390/diagnostics13132289. https://pubmed.ncbi.nlm.nih.gov/37443682/
7. Loginov, Vitaly I, Pronina, Irina V, Filippova, Elena A, Dmitriev, Alexey A, Braga, Eleonora A. 2022. Aberrant Methylation of 20 miRNA Genes Specifically Involved in Various Steps of Ovarian Carcinoma Spread: From Primary Tumors to Peritoneal Macroscopic Metastases. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031300. https://pubmed.ncbi.nlm.nih.gov/35163224/
8. Loginov, V I, Burdennyy, A M, Filippova, E A, Khodyrev, D S, Braga, E A. 2021. Aberrant Methylation of 21 MicroRNA Genes in Breast Cancer: Sets of Genes Associated with Progression and a System of Markers for Predicting Metastasis. In Bulletin of experimental biology and medicine, 172, 67-71. doi:10.1007/s10517-021-05333-x. https://pubmed.ncbi.nlm.nih.gov/34792716/
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