Mir195a-KO Mouse
一般名
Mir195a-KO
製品ID
S-KO-19048
背景情報
C57BL/6JCya
系統ID
KOCMP-387190-Mir195a-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Mir195a-KO Mouse（カタログ番号S-KO-19048）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mir195a-KO
系統ID
KOCMP-387190-Mir195a-B6J-VA
遺伝子名
製品ID
S-KO-19048
遺伝子別名
Mir195, Mirn195, mir-195a, mmu-mir-195, mmu-mir-195a
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000083477
NCBIトランスクリプトID
NR_029581
ターゲット領域
Exon 1
有効領域の大きさ
~0.1 kb
遺伝子研究の概要
Mir195a is a microRNA that plays crucial roles in multiple biological processes. It is involved in regulating cell proliferation, apoptosis, and metabolism, and is associated with pathways like the Hippo signaling pathway. MicroRNAs like Mir195a are important for maintaining normal cellular functions and their dysregulation can lead to various pathological conditions [1,4].
In miR-195a KO mice, cognitive impairment and reduced dendritic spine density were observed, indicating its role in age-associated cognitive functions. Sema3D was identified as a direct target of miR-195a, and its age-dependent increase in rodent brains suggested a possible contribution to age-associated neurodegeneration [2].
In mice prenatally exposed to vinclozolin, increased miR-195a levels in the offspring's penises, testes, uteri, and ovaries were found. This led to activation of the Hippo pathway, down-regulation of the androgen receptor in penises, and ultimately caused penile, testicular, uterine, and ovarian damage and malformations [1,4].
In Dicer-deficient macrophages, restoring miR-195a expression could rescue mitochondrial fatty acid oxidative metabolism, highlighting its role in macrophage activation and atherosclerosis prevention [3]. Also, miR-195a inhibits adipocyte differentiation by targeting Zfp423, suggesting its involvement in obesity and metabolic diseases [5].
In intervertebral disk degeneration, silencing of lncRNA NEAT1 upregulates miR-195a to attenuate the process via the BAX/BAK pathway [6]. Benzothiazole-based conjugates affect miR-195a expression, causing cell cycle arrest and apoptosis in hepatocellular carcinoma cells [7].
In conclusion, Mir195a is essential in various biological processes and disease conditions. Gene knockout mouse models have been instrumental in revealing its functions in age-associated cognitive impairment, reproductive organ development, macrophage-related atherosclerosis, adipocyte differentiation, intervertebral disk degeneration, and hepatocellular carcinoma. Understanding Mir195a provides insights into disease mechanisms and potential therapeutic targets.
References：
1. Yu, Haiming, Yang, Jinru, Zhang, Yujing, Yan, Zhengli, Zhu, Yongfei. 2021. Vinclozolin-induced mouse penile malformation and "small testis" via miR132, miR195a together with the Hippo signaling pathway. In Toxicology, 460, 152842. doi:10.1016/j.tox.2021.152842. https://pubmed.ncbi.nlm.nih.gov/34182078/
2. Chen, Chien-Yuan, Chao, Yung-Mei, Cho, Ching-Chang, Chan, Julie Y H, Juo, Suh-Hang H. 2023. Cerebral Semaphorin3D is a novel risk factor for age-associated cognitive impairment. In Cell communication and signaling : CCS, 21, 140. doi:10.1186/s12964-023-01158-5. https://pubmed.ncbi.nlm.nih.gov/37316917/
3. Wei, Yuanyuan, Corbalán-Campos, Judit, Gurung, Rashmi, Zimmer, Ralf, Schober, Andreas. . Dicer in Macrophages Prevents Atherosclerosis by Promoting Mitochondrial Oxidative Metabolism. In Circulation, 138, 2007-2020. doi:10.1161/CIRCULATIONAHA.117.031589. https://pubmed.ncbi.nlm.nih.gov/29748186/
4. Fu, Hu, Yang, Jinru, Xin, Bingyan, Yan, Zhengli, Zhu, Yongfei. 2023. Accentuated Hippo pathway and elevated miR-132 and miR-195a lead to changes of uteri and ovaries in offspring mice following prenatal exposure to vinclozolin. In Reproductive toxicology (Elmsford, N.Y.), 116, 108335. doi:10.1016/j.reprotox.2023.108335. https://pubmed.ncbi.nlm.nih.gov/36642194/
5. Yun, Ui Jeong, Song, No-Joon, Yang, Dong Kwon, Park, Kye Won, Kang, Hara. . miR-195a inhibits adipocyte differentiation by targeting the preadipogenic determinator Zfp423. In Journal of cellular biochemistry, 116, 2589-97. doi:10.1002/jcb.25204. https://pubmed.ncbi.nlm.nih.gov/25903991/
6. Tang, Ning, Dong, Yulei, Liu, Jiaming, Zhao, Hong. 2020. Silencing of Long Non-coding RNA NEAT1 Upregulates miR-195a to Attenuate Intervertebral Disk Degeneration via the BAX/BAK Pathway. In Frontiers in molecular biosciences, 7, 147. doi:10.3389/fmolb.2020.00147. https://pubmed.ncbi.nlm.nih.gov/32850952/
7. Pushpavalli, Sncvl, Ramaiah, M Janaki, Srinivas, Ch, Bhadra, Utpal, Bhadra, Manika Pal. 2011. Effect of Benzothiazole based conjugates in causing apoptosis by Regulating p53, PTEN and MAP Kinase proteins affecting miR-195a and miR-101-1. In Cancer cell international, 11, 36. doi:10.1186/1475-2867-11-36. https://pubmed.ncbi.nlm.nih.gov/22035408/
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