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6 件の結果が “12307” で取得されました
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Calb1-P2A-iCre
製品ID :
I001156
系統:
C57BL/6JCya
状況:
説明:
The TAG stop codon was replaced with the P2A-iCre cassette. Cre recombinase is expressed under the regulatory control of Calb1 gene elements.
The TAG stop codon was replaced with the P2A-iCre cassette. Cre recombinase is expressed under the regulatory control of Calb1 gene elements.
Calb1-dgCre (DHFR-EGFP-Cre)
製品ID :
C001747
系統:
C57BL/6JCya
状況:
説明:
The TAG stop codon was replaced with the T2A-dgCre (DHFR-EGFP-Cre) cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Calb1 gene elements. The DHFR (dihydrofolate reductase) fragment, derived from the N-terminal 159 amino acids of E. coli ecDHFR, functions as a destabilizing domain (DD). In the absence of specific ligands like TMP (Trimethoprim), this fragment leads to the rapid proteasomal degradation of Cre recombinase. This mechanism allows for the temporal-specific induction of Cre recombinase activity by regulating DHFR-Cre stability with small molecules such as TMP. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.
The TAG stop codon was replaced with the T2A-dgCre (DHFR-EGFP-Cre) cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Calb1 gene elements. The DHFR (dihydrofolate reductase) fragment, derived from the N-terminal 159 amino acids of E. coli ecDHFR, functions as a destabilizing domain (DD). In the absence of specific ligands like TMP (Trimethoprim), this fragment leads to the rapid proteasomal degradation of Cre recombinase. This mechanism allows for the temporal-specific induction of Cre recombinase activity by regulating DHFR-Cre stability with small molecules such as TMP. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.
Calb1-KO
製品ID :
S-KO-18229
系統:
C57BL/6JCya
状況:
説明:
Calb1 is located on chromosome 4 of mice. Nuclease Technology was used to design sgRNA; Calb1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
Calb1 is located on chromosome 4 of mice. Nuclease Technology was used to design sgRNA; Calb1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
Zfand4-KO
製品ID :
S-KO-12307
系統:
C57BL/6JCya
状況:
説明:
Zfand4 is located on chromosome 6 of mice. Nuclease Technology will be used to design sgRNA; Zfand4 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
Zfand4 is located on chromosome 6 of mice. Nuclease Technology will be used to design sgRNA; Zfand4 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
Otor-flox
製品ID :
S-CKO-12307
系統:
C57BL/6JCya
状況:
説明:
Otor is located on chromosome 2 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Otor conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.
Otor is located on chromosome 2 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Otor conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.
Calb1-flox
製品ID :
S-CKO-01505
系統:
C57BL/6JCya
状況:
説明:
Calb1 is located on chromosome 4 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Calb1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
Calb1 is located on chromosome 4 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Calb1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
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