The KRT19 (K19) gene encodes a type I intermediate filament protein (Keratin 19), expressed in various types of epithelial cells from the embryonic layer to adult cells. It is notably expressed in the lungs, oral cavity, bronchi, bronchioles, stomach, and small intestine, as well as in the ductal cells of the pancreas, kidneys, and liver. This protein is a member of the keratin family and plays a crucial role in maintaining the structural integrity of epithelial cells. Deletion of the Krt19 gene can lead to inducible skeletal muscle disease in mice. Additionally, Keratin 19 can be used as a biomarker for detecting tumor cells in various tissues. Depending on the type of cancer cells, regulating the expression of KRT19 can have different impacts on cell proliferation, survival, invasion, migration, and apoptosis ^[1]. The Krt19-iCre mouse model is constructed by knocking a high-recombination activity iCre element (codon-optimized Cre) into the endogenous Krt19 gene in mice. When Krt19-iCre mice are crossed with mice containing loxP sites, Cre recombinase-mediated recombination between loxP sites is expected to occur in KRT19-expressing epithelial cells in the offspring.

9430069I07Rik is located on chromosome 15 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; 9430069I07Rik conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Krt19 is located on chromosome 11 of mice. Nuclease Technology will be used to design sgRNA; Krt19 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Krt19 is located on chromosome 11 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Krt19 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.