The region downstream of the TGA stop codon was replaced with the "IRES-iCreERT2-P2A-EGFP-WPRE-BGH pA" cassette. CreERT2 recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Cd209a gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with mice containing loxP sites, the offspring mice are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in Cd209a-positive cells following Tamoxifen induction. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.

Cd209a is located on chromosome 8 of mice. Nuclease Technology was used to design sgRNA; Cd209a knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Cd209a is located on chromosome 8 of mice. Nuclease Technology was used to design sgRNA; Cd209a knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Cd209a is located on chromosome 8 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Cd209a conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.