Ms4a3 (Membrane spanning 4-domains subfamily A member 3) is a member of the MS4A transmembrane protein family and is primarily expressed in granulocyte-monocyte progenitors (GMPs) and their downstream myeloid cells, including neutrophils, monocytes, eosinophils, and basophils. Studies have shown that Ms4a3 plays important roles in myeloid cell differentiation, hematopoietic development, and immune lineage specification, and has been widely used as a marker gene for myeloid progenitors and their descendant cells ^[1]. In recent years, Ms4a3-related lineage tracing systems have been applied in studies of the developmental origins of neutrophils and monocyte-macrophage lineages, inflammatory responses, and tissue immune microenvironments. Ms4a3-IRES-iCre mice were generated by knocking an IRES-iCre cassette into the endogenous Ms4a3 locus, enabling the expression of codon-optimized iCre recombinase under the control of endogenous Ms4a3 regulatory elements. When crossed with mice containing loxP sites, Cre-mediated recombination between loxP sites is expected to occur in Ms4a3-positive cells of the offspring.

Ms4a3 (Membrane spanning 4-domains subfamily A member 3) is a member of the MS4A transmembrane protein family and is primarily expressed in granulocyte-monocyte progenitors (GMPs) and their downstream myeloid cells, including neutrophils, monocytes, eosinophils, and basophils. Studies have shown that Ms4a3 plays important roles in myeloid cell differentiation, hematopoietic development, and immune lineage specification, and has been widely used as a marker gene for myeloid progenitors and their descendant cells ^[1]. In recent years, Ms4a3-related lineage tracing systems have been applied in studies of the developmental origins of neutrophils and monocyte-macrophage lineages, inflammatory responses, and tissue immune microenvironments. Ms4a3-IRES-tdTomato mice were generated via gene editing by integrating an IRES-tdTomato cassette downstream of the Ms4a3 stop codon, driving tdTomato reporter expression under the control of endogenous Ms4a3 regulatory elements.

Ms4a3 is located on chromosome 19 of mice. Nuclease Technology was used to design sgRNA; Ms4a3 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Ms4a3 is located on chromosome 19 of mice. Nuclease Technology will be used to design sgRNA; Ms4a3 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Ms4a3 is located on chromosome 19 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Ms4a3 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.