The Pvalb gene encodes the protein parvalbumin, a high-affinity calcium-binding protein structurally and functionally similar to calmodulin and troponin C, and is thought to be involved in muscle relaxation. Parvalbumin is primarily expressed in the kidneys and brain, and its expression in the nervous system is fundamental. It participates in excitatory and inhibitory chemical synaptic transmission and maintains normal neural function. Pvalb-T2A-iCre mice were generated using gene editing technology to integrate a T2A-iCre expression cassette into the stop codon of the mouse endogenous Pvalb gene. Under the control of the Pvalb gene's regulatory elements, the iCre recombinase (a codon-optimized Cre recombinase) is expressed. The expression pattern of iCre recombinase is similar to that of the endogenous Pvalb gene. Using the 2A peptide for linkage avoids the drawbacks of low protein activity and low downstream gene expression in multi-gene expression processes. When Pvalb-T2A-iCre mice are crossed with mice containing loxP sites, Cre recombinase-mediated recombination between loxP sites will occur in the neurons expressing parvalbumin protein in the offspring.

Pde3b is located on chromosome 7 of mice. Nuclease Technology was used to design sgRNA; Pde3b knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Cacnb3 is located on chromosome 15 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Cacnb3 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Pvalb is located on chromosome 15 of mice. Nuclease Technology will be used to design sgRNA; Pvalb knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Pvalb is located on chromosome 15 of mice. Nuclease Technology was used to design sgRNA; Pvalb knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Pvalb is located on chromosome 15 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Pvalb conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.