Slc34a1-CreERT2 mice were generated by gene editing technology through insertion of the Kozak-CreERT2-P2A gene expression cassette upstream of the start codon of the mouse Slc34a1 gene. In the absence of tamoxifen treatment, the CreERT2 recombinase is primarily localized in the cytoplasm; only upon tamoxifen administration can the CreERT2 recombinase enter the nucleus and exert its recombinase activity. When Slc34a1-CreERT2 mice are crossed with mice carrying loxP sites, the offspring are expected to undergo Cre recombinase-mediated recombination between loxP sites in renal proximal tubule epithelial cells and Slc34a1-positive cells after tamoxifen induction.

Serpinf2 is located on chromosome 11 of mice. Nuclease Technology was used to design sgRNA; Serpinf2 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Slc34a1 is located on chromosome 13 of mice. Nuclease Technology was used to design sgRNA; Slc34a1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Slc34a1 is located on chromosome 13 of mice. Nuclease Technology was used to design sgRNA; Slc34a1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Slc34a1 is located on chromosome 13 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Slc34a1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.