Toll-like receptor 2 (TLR2) is a transmembrane receptor, encoded by the human TLR2 gene, and is primarily expressed in cell types such as microglia, monocytes, macrophages, and dendritic cells. TLR2 is a member of the Toll-like receptor family, playing an important role in innate immune responses. TLR2 is typically located on the cell surface and forms heterodimers with other Toll-like receptors, such as TLR1 and TLR6, to recognize and respond to different pathogen molecules. TLR2 can recognize pathogen-associated molecular patterns (PAMPs) from many pathogens (such as viruses, bacteria, and fungi), which are composed of proteins, carbohydrates, lipids, nucleic acids, or their combinations. By recognizing different PAMPs, TLR2 can initiate a signal cascade response, thereby promoting immune responses. This strain is a Tlr2 gene knockout (Tlr2 KO) mouse, which uses gene editing technology to knock out the Tlr2 gene in mice homologous to the human TLR2 gene. Homozygous Tlr2 KO mice are viable and fertile. It has been reported that the cells of this mouse have defects in TLR2-mediated signal transduction, causing the mouse to exhibit abnormalities in immune responses against bacterial and viral infections. Therefore, Tlr2 KO is often used to study the role of TLR2 in pathogen recognition and the activation of innate immunity.

Agap3 is located on chromosome 5 of mice. Nuclease Technology will be used to design sgRNA; Agap3 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Tlr2 is located on chromosome 3 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Tlr2 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.