The partial exon 1 coding region was replaced with the Kozak-CreERT2-WPRE-BGH pA cassette. CreERT2 recombinase is expressed under the regulatory control of Wdfy1 gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with mice containing loxP sites, the offspring mice are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in Wdfy1-positive cells following Tamoxifen induction.

Wdfy1 is located on chromosome 1 of mice. Nuclease Technology was used to design sgRNA; Wdfy1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Wdfy1 is located on chromosome 1 of mice. Nuclease Technology will be used to design sgRNA; Wdfy1 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Wdfy1 is located on chromosome 1 of mice. Nuclease Technology was used to design sgRNA; Wdfy1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Wdfy1 is located on chromosome 1 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Wdfy1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.