Ubiquitin-Specific Protease 26 (USP26), which belongs to the ubiquitination-specific processing protease family, is a deubiquitinating enzyme with histidine (His) and cysteine (Cys) structural domains. the USP26 gene is specifically expressed in testicular tissue as well as in the blood-testis barrier, and premeiotic spermatogonia during spermatogenesis The gene is expressed in the spermatogonia before meiosis during spermatogenesis, including primitive A-, Ap- and B-type spermatogonia. The main diseases associated with USP26 are male infertility, including weak teratozoospermia, non-obstructive azoospermia (NOA), Sertoli cell-only syndrome (SCOS), and Klinefelter syndrome (KS). This model is a Usp26 gene-knockout mouse. Homozygous mice of this strain are viable.

Usp26 is located on chromosome X of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Usp26 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.