The Lyz1 gene encodes lysozyme C, a critical enzyme predominantly expressed in embryonic mesenchyme, intestine, liver, biliary system, and lungs. In mice, Paneth cells, specialized epithelial cells residing in the small intestine, serve as the principal source of lysozyme C. Studies have demonstrated that the targeted disruption of Lyz1 provides protection against experimental colitis. Loss of Lyz1 impairs the intestinal immune response to bacterial molecular patterns, thereby promoting the expansion of lysozyme-sensitive, mucus-dissolving bacterial populations ^[1]. The Lyz1-H2BmCherry-IRES-CreERT2 mice are constructed by knocking the H2BmCherry-IRES-CreERT2-rBG pA cassette into the mouse Lyz1 gene, a strategy that disrupts the expression of the endogenous Lyz1 gene. Without tamoxifen treatment, CreERT2 recombinase remains in the cytoplasm. After tamoxifen treatment, CreERT2 recombinase can enter the nucleus and exert its recombination function. When Lyz1-H2BmCherry-IRES-CreERT2 mice are crossed with mice containing loxP sites, tamoxifen-induced offspring will undergo Cre recombinase-mediated sequence recombination in Paneth cells of the small intestine.

Olfm4 is located on chromosome 14 of mice. Nuclease Technology was used to design sgRNA; Olfm4 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Hamp is located on chromosome 7 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Hamp conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Lyz1 is located on chromosome 10 of mice. Nuclease Technology was used to design sgRNA; Lyz1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Lyz1 is located on chromosome 10 of mice. Nuclease Technology was used to design sgRNA; Lyz1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Lyz1 is located on chromosome 10 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Lyz1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.