Lyz2-IRES-CreERT2 Mouse
製品名
Lyz2-IRES-CreERT2 Mouse
製品ID
C001499
系統名
C57BL/6JCya-Lyz2em3(IRES-CreERT2)/Cya
背景情報
C57BL/6JCya
組織や細胞を表現する例
Bone marrow cell lineages (monocytes, mature macrophages, and granulocytes)
状況
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製品タイプ
年齢
遺伝子型
性別
数量
遺伝子名
遺伝子別名
Lys, Lzm, Lzp, Lysm, Lyzs, Lyzf2, Lzm-s1
NCBI ID
染色体
Chr 10
MGI ID
さらに
系統詳細
Lysozyme, encoded by the LYZ gene, is an integral component of the innate immune system. Its primary function is to disrupt bacterial cell walls by cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan, leading to bacterial lysis. Lysozyme is abundant in various secretions, including tears, saliva, human milk, and mucus, as well as in the cytoplasmic granules of macrophages and polymorphonuclear neutrophils. Cytoplasmic lysozyme expression is high in bone marrow cells, Paneth cells, and a subset of salivary gland cells [1-2].
Mice have two lysozyme-encoding genes, Lyz1 and Lyz2, which are highly homologous to the human LYZ gene. Lyz1 is primarily expressed in Paneth cells, while Lyz2 is mainly expressed in myeloid cells. Myeloid-specific expression of Lyz2 is associated with cell-type-specific demethylation of its downstream enhancer. The Lyz2 protein also has lysozyme activity and participates in defense responses against both Gram-negative and Gram-positive bacteria. Lyz2 is expressed in the hematopoietic system, liver, mesothelium, small intestine, and yolk sac of mice [3-4].
Lyz2-IRES-CreERT2 mice were generated using gene editing technology to integrate the tamoxifen-inducible CreERT2 recombinase expression element into the 3’UTR of the mouse Lyz2 gene. This mimics the expression pattern of the endogenous gene while maintaining Lyz2 expression. When bred with mice carrying a loxP site-flanked sequence, Cre recombinase-mediated recombination of the flanked sequence occurs in myeloid cells, including monocytes, mature macrophages, and granulocytes, following tamoxifen induction.
参考文献
Yoshimura K, Toibana A, Nakahama K. Human lysozyme: sequencing of a cDNA, and expression and secretion by Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1988 Jan 29;150(2):794-801.
Sharon N. The chemical structure of lysozyme substrates and their cleavage by the enzyme. Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):402-15.
Peters CW, Kruse U, Pollwein R, Grzeschik KH, Sippel AE. The human lysozyme gene. Sequence organization and chromosomal localization. Eur J Biochem. 1989 Jul 1;182(3):507-16.
Clausen BE, Burkhardt C, Reith W, Renkawitz R, Förster I. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 1999 Aug;8(4):265-77.
系統作製戦略
The IRES-CreERT2 gene expression element was integrated into the 3'UTR region of the mouse Lyz2 gene using gene editing technology.
Figure 1. Diagram of the gene editing strategy for the generation of Lyz2-IRES-CreERT2 mice.
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