RCL-tdTomato Mouse

Figure 2. Expression of tdTomato protein in peripheral blood (PB) B lymphocytes (mCD19+) and T lymphocytes (mCD3+). FACS analysis showed extremely strong tdTomato expression in PB B lymphocytes of Cre+ mice, with a recombination efficiency of ~98% in B lymphocytes. In contrast, minimal Cre recombination was detected in PB T lymphocytes.

Figure 3. Expression of tdTomato protein in splenic B lymphocytes (mCD19+) and T lymphocytes (mCD3+). FACS results revealed extremely strong tdTomato expression in splenic B lymphocytes of Cre+ mice, with a recombination efficiency of ~88% in B cells. Weak Cre recombination was detected in splenic T lymphocytes of Cre+ mice, potentially linked to nonspecific Cre recombinase expression or expression leakage. Internal data showed that RCL-tdTomato[CKI/+];Cd19-iCre[KI/+] mice exhibited lower recombination efficiency in splenic T lymphocytes compared to Rosa26-LSL-tdTomato[CKI/+];Cd19-iCre[KI/+] mice. Given that Cd19-iCre mice are B lymphocyte-specific Cre mice, these results further indicate that RCL-tdTomato mice may be more suitable than Rosa26-LSL-tdTomato mice (Product No.: C001476) for detecting Cre mice with high recombination efficiency and potential expression leakage.

Figure 4. Expression of tdTomato protein in bone marrow (BM) B lymphocytes (mCD19+). FACS analysis showed extremely strong tdTomato expression in BM B lymphocytes of Cre+ mice, with a recombination efficiency of 86% ~ 90% in B cells.