B6-huIL15 Mouse
製品名
B6-huIL15 Mouse
製品ID
C001853
系統名
C57BL/6NCya-Il15tm1(hIL15)/Cya
背景情報
C57BL/6NCya
状況
このマウス系統を論文で使用する場合は、「B6-huIL15 Mouse（カタログ番号C001853）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
遺伝子名
遺伝子別名
IL-15
NCBI ID
染色体
Chr 4
MGI ID
さらに
系統詳細
The IL15 gene encodes a pleiotropic four-α-helix bundle cytokine known as Interleukin-15 (IL-15), which is essential for the development, survival, and activation of immune cells, particularly Natural Killer (NK) cells and memory CD8+ T cells. Unlike many cytokines, IL-15 is primarily regulated at the post-transcriptional and translational levels rather than just transcriptionally, and it is uniquely delivered to target cells through trans-presentation, where it is shuttled to the cell surface bound to its high-affinity receptor, IL-15Rα [1]. The protein is widely expressed across a variety of tissues, including the placenta, skeletal muscle, kidney, lung, and heart, and is produced by both hematopoietic cells (such as monocytes, macrophages, and dendritic cells) and non-hematopoietic cells (such as epithelial cells and fibroblasts) [2]. Functionally, IL-15 triggers the JAK/STAT (specifically JAK1/3 and STAT3/5) and PI3K/AKT/mTOR signaling pathways to promote cellular proliferation and inhibit apoptosis by upregulating anti-apoptotic factors like BCL2 [3]. Because of its potent inflammatory effects, dysregulation of the IL15 gene is implicated in several pathologies: over-expression is strongly associated with autoimmune diseases like Celiac disease, Rheumatoid Arthritis, and Multiple Sclerosis, as well as certain malignancies like Adult T-cell Leukemia, while its deficiency can lead to severe immunodeficiency or impaired response to viral infections [4].
The B6-huIL15 mouse is a humanized model constructed through gene-editing technology, in which the region from partial intron 4 to TGA stop codon of mouse Il15 is replaced with the region from partial intron 4 to TGA stop codon of human IL15. This model can be used for research on autoimmune diseases like Celiac disease, Rheumatoid Arthritis, and Multiple Sclerosis, as well as certain malignancies like Adult T-cell Leukemia. Furthermore, it serves as a platform for the screening, development, and preclinical evaluation of IL15-targeted therapeutics.
参考文献
Fehniger TA, Caligiuri MA. Interleukin 15: biology and relevance to human disease. Blood. 2001 Jan 1;97(1):14-32.
Perera PY, Lichy JH, Waldmann TA, Perera LP. The role of interleukin-15 in inflammation and immune responses to infection: implications for its therapeutic use. Microbes Infect. 2012 Mar;14(3):247-61.
Mishra A, Sullivan L, Caligiuri MA. Molecular pathways: interleukin-15 signaling in health and in cancer. Clin Cancer Res. 2014 Apr 15;20(8):2044-50.
Meghnem D, Maillasson M, Barbieux I, Morisseau S, Keita D, Jacques Y, Quéméner A, Mortier E. Selective Targeting of IL-15Rα Is Sufficient to Reduce Inflammation. Front Immunol. 2022 May 3;13:886213.
系統作製戦略
The region from partial intron 4 to TGA stop codon of mouse Il15 was replaced with the region from partial intron 4 to TGA stop codon of human IL15.
Figure 1. Diagram of the gene editing strategy for the generation of B6-huIL15 mice.
適用分野
Screening, development and pre-clinical evaluation of IL15-targeted drugs;
Research on autoimmune diseases, such as celiac disease, rheumatoid arthritis, and multiple sclerosis;
Research on the pathogenic mechanisms and related treatment methods of malignant tumors, such as adult T-cell leukemia.
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