hIGF1R/huTFRC/huCD98HC Mouse

Figure 1. Diagram of the gene editing strategy for the generation of hIGF1R mice. Part of exon 1 coding region and part of intron 1 of the mouse Igf1r gene were replaced by the Human IGF1R CDS*-Mouse Igf1r CDS**-WPRE-BGH pA sequence. The signal peptide of the mouse was retained. *Human IGF1R CDS: Alpha chain and beta chain (including its extracellular domain). **Mouse Igf1r CDS: Transmembrane domain and cytoplasmic domain.

Figure 2. Gene editing strategy of huTFRC mice. The mouse Tfrc endogenous extracellular domain was replaced with the human TFRC extracellular domain. The murine cytoplasmic and helical regions were kept.

Figure 3. Gene editing strategy of huCD98HC(SLC3A2) mice. The mouse Slc3a2 endogenous extracellular domain was replaced with the human SLC3A2 extracellular domain. The murine cytoplasmic domain and transmembrane domain were preserved.