RCL-Mito-mKate2 Mouse

This mouse model carries a reporter gene encoding the mitochondria-targeted far-red fluorescent protein Mito-mKate2 for conditional expression. The reporter gene is driven by the CAG promoter and conditionally blocked by a loxP-Stop-loxP (LSL) element. Following Cre-mediated excision of the LSL element, Mito-mKate2 expression is driven by the CAG promoter. Mito-mKate2 is specifically targeted to mitochondria through its N-terminal fused mitochondrial targeting sequence (Mito). mKate2 is a monomeric far-red fluorescent protein derived from the fluorescent protein family of Entacmaea quadricolor and is an improved variant of mKate. It has a typical maximum excitation wavelength of approximately 588 nm and a maximum emission wavelength of approximately 633 nm ^[1].

RCL-Mito-mKate2 mice were generated by integrating the CAG promoter-loxP-PGK-Neo-6×SV40 pA-loxP-Kozak-Mito-mKate2-WPRE-BGH pA expression cassette into intron 1 of the mouse Rosa26 gene on chromosome 6 using gene-editing technology. The mKate2 protein can specifically localize to mitochondria within cells for conditional expression.