Angptl4-P2A-DreERT2 Mouse

Angptl4 (angiopoietin-like 4) encodes angiopoietin-like protein 4 (ANGPTL4), a secreted glycoprotein of the angiopoietin-like family that inhibits lipoprotein lipase (LPL) activity and participates in the partitioning and metabolic regulation of plasma triglycerides ^[1]. Angptl4 is a target gene of peroxisome proliferator-activated receptors (PPARs); it is expressed in adipose tissue and can be induced by fasting and by PPAR agonists ^[2]. The gene has been reported to be expressed relatively abundantly in adipocytes and hepatocytes, and liver-derived ANGPTL4 contributes to the partitioning of circulating triglycerides among tissues ^[1,3].

Angptl4-P2A-DreERT2 mice were generated by gene editing, in which the stop codon of the endogenous mouse Angptl4 gene was replaced with the "P2A-DreERT2" cassette. In the absence of tamoxifen treatment, the DreERT2 recombinase is mainly localized in the cytoplasm; after tamoxifen treatment, the DreERT2 recombinase enters the nucleus and exerts its recombination function. When Angptl4-P2A-DreERT2 mice are crossed with mice carrying rox sites, the offspring are expected to undergo Dre recombinase-mediated recombination between rox sites in Angptl4-positive cells after tamoxifen induction.