RCL-CNA35-mCherry Mouse
製品名
RCL-CNA35-mCherry Mouse
製品ID
C002228
系統名
C57BL/6Cya-Gt(ROSA)26Sorem1(CAG-LSL-CNA35-mCherry)/Cya
背景情報
C57BL/6Cya
状況
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製品タイプ
年齢
遺伝子型
性別
数量
系統詳細
The Rosa26 (Gt(ROSA)26Sor) locus is one of the most widely used integration sites for foreign sequences in the mouse; it shows broad transcriptional activity in the embryo and in many adult tissues, so reporter elements integrated there can be expressed in a wide range of cell types [1,2]. A CAG promoter-driven loxP-STOP-loxP (RCL) configuration is a commonly used Cre-dependent reporter design: before recombination the blocking sequence suppresses downstream expression, and after Cre-mediated recombination the CAG promoter drives the downstream sequence [3]. CNA35 is the collagen-binding domain of the collagen adhesin (Cna) of Staphylococcus aureus, binds collagen specifically and has been used for fluorescent labelling of collagen [4,5]; mCherry is a red fluorescent protein.
RCL-CNA35-mCherry mice were generated by integrating the "CAG promoter-loxP-PGK-Neo-6xSV40 pA-loxP-Kozak-CNA35-mCherry-WPRE-BGH pA" expression cassette into the Rosa26 locus. The model can be used for Cre/loxP-mediated conditional gene recombination and for red fluorescent labelling of recombined cells.
参考文献
Zambrowicz BP, Imamoto A, Fiering S, Herzenberg LA, Kerr WG, Soriano P. Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proceedings of the National Academy of Sciences of the United States of America. 1997;94(8):3789-3794. doi:10.1073/pnas.94.8.3789.
Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nature Genetics. 1999;21(1):70-71. doi:10.1038/5007.
Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nature Neuroscience. 2010;13(1):133-140. doi:10.1038/nn.2467.
de Jong S, van Middendorp LB, Hermans RH, de Bakker JM, Bierhuizen MF, Prinzen FW, van Rijen HV, Losen M, Vos MA, van Zandvoort MA. Ex vivo and in vivo administration of fluorescent CNA35 specifically marks cardiac fibrosis. Molecular Imaging. 2014;13:1-8. doi:10.2310/7290.2014.00036.
Pokidysheva EN, Sales JD, Yagi S, Ueno T, Sasai K, Makarenko A, Bächinger HP, Mizuno K, Boudko SP. The staphylococcal collagen adhesin CNA35 effectively detects collagen and its fragments in blots after SDS-PAGE. Matrix Biology Plus. 2025;26:100174. doi:10.1016/j.mbplus.2025.100174.
系統作製戦略
The "CAG promoter-loxP-PGK-Neo-6xSV40 pA-loxP-Kozak-CNA35-mCherry-WPRE-BGH pA" cassette was cloned into intron 1 of the Rosa26 gene.
Figure 1. Gene editing strategy of RCL-CNA35-mCherry mice.
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