TG-CAG-mito-mNeonGreen Mouse

This strain is a fluorescently labeled model carrying a constitutively fluorescent green/yellow protein (mNeonGreen) reporter gene expression element, with mNeonGreen protein expression regulated by the CAG promoter. The CAG promoter is constructed by modifying and integrating the chicken β-actin gene promoter, the cytomegalovirus (CMV) early enhancer, the rabbit β-actin gene splice acceptor, and the bovine globin polyA signal. It is renowned for its strong and stable real-time gene expression in various mammalian cells, making it a popular choice for driving gene expression in transgenic models. The mNeonGreen protein is a monomeric green/yellow fluorescent protein derived from the lanthanoid bronchitis virus, and it can be used as a fusion tag for conventional imaging and stochastic single-molecule super-resolution imaging. It is also an excellent fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent protein ^[1]. This strain is constructed by integrating the CAG-mito-mNeonGreen gene expression cassette into the mouse genome using transgenic technology, resulting in the specific expression of the mNeonGreen protein in mitochondria.