Unc119b-flox Mouse
一般名
Unc119b-flox
製品ID
S-CKO-00528
背景情報
C57BL/6JCya
系統ID
CKOCMP-106840-Unc119b-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Unc119b-flox Mouse（カタログ番号S-CKO-00528）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Unc119b-flox
系統ID
CKOCMP-106840-Unc119b-B6J-VA
遺伝子名
製品ID
S-CKO-00528
遺伝子別名
-
遺伝子別名
C57BL/6JCya
遺伝子正式名称
unc-119 lipid binding chaperone B
NCBI ID
修正
Conditional knockout
染色体
Chr 5
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000060798
NCBIトランスクリプトID
NM_175352
ターゲット領域
Exon 3~5
有効領域の大きさ
~2.4 kb
遺伝子研究の概要
Unc119b is one of the paralogs of the guanine dissociation inhibitor-like solubilizing factors UNC119 in the human genome. It binds to N-myristoylated proteins, facilitating their trafficking between different membranes, and is involved in the shuttling and sorting of lipid-modified proteins to the primary cilium [2,4,5]. It may also play a role in insulin sensitivity as Unc119 proteins were identified as targets of compounds that enhance insulin-stimulated GLUT4 translocation [3].
In hepatocellular carcinoma (HCC), a Cox model including Unc119b suggested unfavorable overall survival in the high-risk score group. Unc119b, along with NR6A1 and OSBP2, could inhibit NK cell and TIL cell infiltration, and impair Type-I and II IFN responses in HCC, contributing to poor prognosis and immunotherapy resistance. Unc119b was also related to chemotherapy resistance [1].
In zebrafish, knockdown of unc119b orthologs led to visual impairment and early-onset retinal dystrophy, indicating its importance in cone function [6]. In cep290-deficient zebrafish, upregulation of unc119b was associated with milder phenotypes, suggesting a role in genetic compensation for cilia defects [7].
Low-dose ionizing radiation led to promoter hypermethylation and transcriptional downregulation of Unc119b in endothelial cells, making it a potential DNA methylation biomarker for cardiovascular risk assessment [8].
In conclusion, Unc119b is crucial for the trafficking of myristoylated proteins, especially in ciliary processes. Its role in diseases such as HCC, as revealed through model-based research, indicates its potential as a therapeutic target. The findings from zebrafish models also highlight its significance in visual function and cilia-related genetic compensation. Additionally, its response to low-dose radiation in endothelial cells shows promise as a biomarker for cardiovascular risk [1,6,7,8].
References：
1. Wu, Qinjuan, Li, Xin, Long, Menghong, Xie, Xianfeng, Liu, Qing. 2023. Integrated analysis of histone lysine lactylation (Kla)-specific genes suggests that NR6A1, OSBP2 and UNC119B are novel therapeutic targets for hepatocellular carcinoma. In Scientific reports, 13, 18642. doi:10.1038/s41598-023-46057-4. https://pubmed.ncbi.nlm.nih.gov/37903971/
2. Yelland, Tamas, Garcia, Esther, Samarakoon, Youhani, Ismail, Shehab. 2021. The Structural and Biochemical Characterization of UNC119B Cargo Binding and Release Mechanisms. In Biochemistry, 60, 1952-63. doi:10.1021/acs.biochem.1c00251. https://pubmed.ncbi.nlm.nih.gov/34130453/
3. Yin, Terry C, Van Vranken, Jonathan G, Srivastava, Dhiraj, Gygi, Steven P, Sebag, Julien A. 2023. Insulin sensitization by small molecules enhancing GLUT4 translocation. In Cell chemical biology, 30, 933-942.e6. doi:10.1016/j.chembiol.2023.06.012. https://pubmed.ncbi.nlm.nih.gov/37453421/
4. Constantine, Ryan, Zhang, Houbin, Gerstner, Cecilia D, Frederick, Jeanne M, Baehr, Wolfgang. 2012. Uncoordinated (UNC)119: coordinating the trafficking of myristoylated proteins. In Vision research, 75, 26-32. doi:10.1016/j.visres.2012.08.012. https://pubmed.ncbi.nlm.nih.gov/23000199/
5. Stephen, Louise A, Ismail, Shehab. . Shuttling and sorting lipid-modified cargo into the cilia. In Biochemical Society transactions, 44, 1273-1280. doi:. https://pubmed.ncbi.nlm.nih.gov/27911709/
6. Rainy, Nir, Etzion, Talya, Alon, Shahar, Gothilf, Yoav, Stiebel-Kalish, Hadas. 2016. Knockdown of unc119c results in visual impairment and early-onset retinal dystrophy in zebrafish. In Biochemical and biophysical research communications, 473, 1211-1217. doi:10.1016/j.bbrc.2016.04.041. https://pubmed.ncbi.nlm.nih.gov/27079236/
7. Cardenas-Rodriguez, Magdalena, Austin-Tse, Christina, Bergboer, Judith G M, Sayer, John A, Drummond, Iain A. 2021. Genetic compensation for cilia defects in cep290 mutants by upregulation of cilia-associated small GTPases. In Journal of cell science, 134, . doi:10.1242/jcs.258568. https://pubmed.ncbi.nlm.nih.gov/34155518/
8. Park, Jihye, Lee, Hae-June, Han, Yu Kyeong, Kang, Keunsoo, Yi, Joo Mi. 2024. Identification of DNA methylation biomarkers for evaluating cardiovascular disease risk from epigenome profiles altered by low-dose ionizing radiation. In Clinical epigenetics, 16, 19. doi:10.1186/s13148-024-01630-0. https://pubmed.ncbi.nlm.nih.gov/38303056/
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