Bex2-flox Mouse
一般名
Bex2-flox
製品ID
S-CKO-01411
背景情報
C57BL/6JCya
系統ID
CKOCMP-12069-Bex2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Bex2-flox Mouse（カタログ番号S-CKO-01411）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Bex2-flox
系統ID
CKOCMP-12069-Bex2-B6J-VA
遺伝子名
製品ID
S-CKO-01411
遺伝子別名
Bex1, REX-3
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr X
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000049130
NCBIトランスクリプトID
NM_009749
ターゲット領域
Exon 3
有効領域の大きさ
~1.9 kb
遺伝子研究の概要
Bex2, or Brain Expressed X-linked gene 2, was originally identified for promoting transcription by interacting with transcription factors in DNA-binding complexes. It is involved in multiple cellular processes and associated with several signaling pathways such as NF-κB, c-Jun/JNK, and ErbB2 pathways [2]. Bex2 has been shown to play a significant role in carcinogenesis, making it biologically important in understanding cancer development [2]. Genetic models like gene knockout (KO) could potentially be valuable in further elucidating its functions.
Knockdown of Bex2 in various cancer cell lines has revealed its role in cancer-related processes. In lung cancer cells, crotonylated Bex2 enhances mitophagy by facilitating the interaction between NDP52 and LC3B, and its overexpression inhibits chemotherapy-induced apoptosis [1]. In breast, colorectal, hepatocellular cancers, and brain tumors, Bex2 promotes cell growth, survival, migration, and invasion, and regulates apoptosis and the cell cycle through cross-talk with relevant signaling pathways [2]. In gastric and hepatocellular carcinomas, Bex2 is associated with cancer stemness and poor prognosis, and its knockdown decreases stem-like properties and increases drug sensitivity [3,4]. In colorectal cancer, Bex2 promotes cell proliferation via the JNK/c-Jun pathway [5]. In intrahepatic cholangiocarcinoma, Bex2 is essential for maintaining dormant cancer stem cells by suppressing mitochondrial activity [6]. In glioma cells, Bex2 promotes migration and invasion, likely mediated by N-cadherin and MMP-2 [7].
In conclusion, Bex2 is a gene with diverse functions in cancer biology, mainly promoting cancer-related processes such as cell growth, survival, migration, and invasion, and is associated with cancer stemness and poor prognosis in multiple cancer types. The use of knockdown and overexpression models in various cancer cell lines has significantly contributed to understanding Bex2's role in these disease conditions, providing potential therapeutic targets for cancer treatment.
References：
1. Mu, Ning, Wang, Yu, Li, Xiaopeng, Su, Ling, Liu, Xiangguo. 2023. Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells. In Cell death & disease, 14, 645. doi:10.1038/s41419-023-06164-6. https://pubmed.ncbi.nlm.nih.gov/37777549/
2. Naderi, Ali. 2019. Molecular functions of brain expressed X-linked 2 (BEX2) in malignancies. In Experimental cell research, 376, 221-226. doi:10.1016/j.yexcr.2019.02.014. https://pubmed.ncbi.nlm.nih.gov/30779920/
3. Yasumoto, Akihiro, Fujimori, Haruna, Mochizuki, Mai, Shibata, Chikashi, Tamai, Keiichi. 2023. BEX2 is poor prognostic factor and required for cancer stemness in gastric cancer. In Biochemical and biophysical research communications, 655, 59-67. doi:10.1016/j.bbrc.2023.03.025. https://pubmed.ncbi.nlm.nih.gov/36933308/
4. Fukushi, Daisuke, Shibuya-Takahashi, Rie, Mochizuki, Mai, Satoh, Kennichi, Tamai, Keiichi. 2021. BEX2 is required for maintaining dormant cancer stem cell in hepatocellular carcinoma. In Cancer science, 112, 4580-4592. doi:10.1111/cas.15115. https://pubmed.ncbi.nlm.nih.gov/34424582/
5. Hu, Yeting, Xiao, Qian, Chen, Haiyan, Yuan, Ying, Ding, Kefeng. 2017. BEX2 promotes tumor proliferation in colorectal cancer. In International journal of biological sciences, 13, 286-294. doi:10.7150/ijbs.15171. https://pubmed.ncbi.nlm.nih.gov/28367093/
6. Tamai, Keiichi, Nakamura-Shima, Mao, Shibuya-Takahashi, Rie, Sugamura, Kazuo, Satoh, Kennichi. 2020. BEX2 suppresses mitochondrial activity and is required for dormant cancer stem cell maintenance in intrahepatic cholangiocarcinoma. In Scientific reports, 10, 21592. doi:10.1038/s41598-020-78539-0. https://pubmed.ncbi.nlm.nih.gov/33299012/
7. Zhou, Xiuping, Xu, Xuebin, Meng, Qingming, Shi, Qiong, Yu, Rutong. 2012. Bex2 is critical for migration and invasion in malignant glioma cells. In Journal of molecular neuroscience : MN, 50, 78-87. doi:10.1007/s12031-012-9864-8. https://pubmed.ncbi.nlm.nih.gov/22907646/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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