Ctsb-flox Mouse
一般名
Ctsb-flox
製品ID
S-CKO-01934
背景情報
C57BL/6JCya
系統ID
CKOCMP-13030-Ctsb-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ctsb-flox Mouse（カタログ番号S-CKO-01934）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ctsb-flox
系統ID
CKOCMP-13030-Ctsb-B6J-VA
遺伝子名
製品ID
S-CKO-01934
遺伝子別名
CB
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 14
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000006235
NCBIトランスクリプトID
NM_007798
ターゲット領域
Exon 3
有効領域の大きさ
~0.7 kb
遺伝子研究の概要
Ctsb, also known as Cathepsin B, is a cysteine protease. It is mainly located in lysosomes and participates in various cellular processes such as protein degradation and antigen processing. It is involved in multiple pathways including the mitochondrial apoptosis pathway, mitophagy-ROS-NLRP3 pathway, and is related to the function of osteoclasts, immune cells infiltration, and autophagy [1-10].
In sepsis-induced acute kidney injury (S-AKI), Ctsb activation via lysosomal membrane permeabilization (LMP) promotes apoptosis through the mitochondrial apoptosis pathway, and its inhibition may be a new treatment strategy [1]. In high-fat-diet-induced hepatic pyroptosis, apigenin alleviates pyroptosis by reducing Ctsb release caused by LMP through the mitophagy-ROS-CTSB-NLRP3 pathway [2]. CST6 protein and peptides can inhibit breast cancer bone metastasis by suppressing Ctsb activity and osteoclastogenesis [3]. In AML, knockdown of Ctsb in HL-60 cells inhibits cell proliferation and tumorigenesis, and may serve as an independent prognostic factor [4]. In gliomas, Ctsb is a negative prognostic biomarker related to immune cells infiltration and immunosuppression [5]. In late middle-aged adults at risk for Alzheimer's disease, aerobic exercise training increases plasma Ctsb, and changes in Ctsb correlate with cognitive performance [6]. In mice, running-induced systemic Ctsb secretion is associated with memory function, and Ctsb knockout mice do not show exercise-enhanced adult hippocampal neurogenesis and spatial memory function [7]. In necroptosis, MLKL polymerization-induced LMP leads to Ctsb release and cell death, and chemical inhibition or knockdown of Ctsb protects cells from necroptosis [8]. Melittin treatment suppresses malignant NSCLC progression through enhancing Ctsb-mediated hyperautophagy [9].
In conclusion, Ctsb is involved in multiple biological processes and diseases. Studies using gene knockout or knockdown models have revealed its role in apoptosis, pyroptosis, cancer metastasis, cell proliferation, cognitive function, and necroptosis. These findings provide insights into potential therapeutic strategies for related diseases.
References：
1. Wang, Yuting, Xi, Wenjie, Zhang, Xinyi, Zheng, Xiaoming, Chi, Xinjin. 2023. CTSB promotes sepsis-induced acute kidney injury through activating mitochondrial apoptosis pathway. In Frontiers in immunology, 13, 1053754. doi:10.3389/fimmu.2022.1053754. https://pubmed.ncbi.nlm.nih.gov/36713420/
2. Meng, Zhuoqun, Gao, Min, Wang, Chunyun, Zhang, DuoDuo, Lu, Jing. 2023. Apigenin Alleviated High-Fat-Diet-Induced Hepatic Pyroptosis by Mitophagy-ROS-CTSB-NLRP3 Pathway in Mice and AML12 Cells. In Journal of agricultural and food chemistry, 71, 7032-7045. doi:10.1021/acs.jafc.2c07581. https://pubmed.ncbi.nlm.nih.gov/37141464/
3. Li, Xiaoxun, Liang, Yajun, Lian, Cheng, Liu, Tong, Hu, Guohong. 2021. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. In Theranostics, 11, 9821-9832. doi:10.7150/thno.62187. https://pubmed.ncbi.nlm.nih.gov/34815788/
4. Peng, Sida, Yang, Qingqing, Li, Huan, Hu, Pan, Zhang, Nana. 2021. CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells. In International journal of medical sciences, 18, 1484-1491. doi:10.7150/ijms.54206. https://pubmed.ncbi.nlm.nih.gov/33628106/
5. Ma, Kaiming, Chen, Xin, Liu, Weihai, Yang, Chenlong, Yang, Jun. 2022. CTSB is a negative prognostic biomarker and therapeutic target associated with immune cells infiltration and immunosuppression in gliomas. In Scientific reports, 12, 4295. doi:10.1038/s41598-022-08346-2. https://pubmed.ncbi.nlm.nih.gov/35277559/
6. Gaitán, Julian M, Moon, Hyo Youl, Stremlau, Matthew, Okonkwo, Ozioma C, van Praag, Henriette. 2021. Effects of Aerobic Exercise Training on Systemic Biomarkers and Cognition in Late Middle-Aged Adults at Risk for Alzheimer's Disease. In Frontiers in endocrinology, 12, 660181. doi:10.3389/fendo.2021.660181. https://pubmed.ncbi.nlm.nih.gov/34093436/
7. Moon, Hyo Youl, Becke, Andreas, Berron, David, Duzel, Emrah, van Praag, Henriette. 2016. Running-Induced Systemic Cathepsin B Secretion Is Associated with Memory Function. In Cell metabolism, 24, 332-40. doi:10.1016/j.cmet.2016.05.025. https://pubmed.ncbi.nlm.nih.gov/27345423/
8. Liu, Shuzhen, Perez, Preston, Sun, Xue, Mammadova, Jamila, Wang, Zhigao. 2023. MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis. In Cell death and differentiation, 31, 40-52. doi:10.1038/s41418-023-01237-7. https://pubmed.ncbi.nlm.nih.gov/37996483/
9. Wang, Yuhan, Yuan, Tailei, He, Longyue, Huang, Gang, Lu, Changlian. 2024. Melittin treatment suppressed malignant NSCLC progression through enhancing CTSB-mediated hyperautophagy. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 180, 117573. doi:10.1016/j.biopha.2024.117573. https://pubmed.ncbi.nlm.nih.gov/39426282/
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精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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