Hsph1-flox Mouse
一般名
Hsph1-flox
製品ID
S-CKO-02991
背景情報
C57BL/6JCya
系統ID
CKOCMP-15505-Hsph1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Hsph1-flox Mouse（カタログ番号S-CKO-02991）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Hsph1-flox
系統ID
CKOCMP-15505-Hsph1-B6J-VA
遺伝子名
製品ID
S-CKO-02991
遺伝子別名
105kDa, Hsp105, Hsp110, hsp-E7I, hsp110/105
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 5
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000202361
NCBIトランスクリプトID
NM_013559
ターゲット領域
Exon 2
有効領域の大きさ
~2.1 kb
遺伝子研究の概要
HSPH1, also known as heat shock 105kDa/110kDa protein 1, is a member of the heat shock protein family. Heat shock proteins play crucial roles in maintaining protein homeostasis, such as preventing protein aggregation, assisting in protein folding, and participating in stress response pathways [5,8].
In cancer research, HSPH1 has been implicated in multiple types of cancers. In gastric cancer, it was identified as a target of ATF2. HSPH1 can interact with SLC7A11 to increase its protein stability, and knockdown of HSPH1 partly reversed the inhibitory effect of ATF2 overexpression on sorafenib-induced ferroptosis [1]. In advanced lung adenocarcinoma, the rs2280059 genetic variant of HSPH1 modulated its expression, and silencing HSPH1 significantly increased the antineoplastic effects of gefitinib [2]. In aggressive B-cell non-Hodgkin lymphoma, HSPH1 silencing downregulated Bcl-6 and c-Myc, hampering the growth of lymphoma cells both in vitro and in vivo [6]. In prostate cancer, HSPH1 may promote the differentiation of conventional T cells towards Th1 or Th2 cells, increase CD8 + T cells in the tumor area, and inhibit prostate cancer invasion [3]. In addition, in N-methyl-N-nitrosourea-induced gastric carcinogenesis, YTHDF1 regulated the translation of HSPH1, affecting malignant transformation and tumorigenesis [4].
In acute lung injury, the IL-1β/KLF2/HSPH1 pathway was activated, facilitating STAT3 phosphorylation in alveolar macrophages and exacerbating pulmonary inflammation [7]. Also, HSPH1 was found as a potential biomarker in doxorubicin-induced chronic cardiotoxicity [9].
In conclusion, HSPH1 is an important gene involved in protein-related cellular functions and stress responses. Its dysregulation is associated with various diseases, especially cancers. Studies using gene-knockdown or other loss-of-function models have revealed its roles in promoting cancer cell growth, drug resistance, and influencing disease-related signaling pathways, providing potential therapeutic targets for these diseases.
References：
1. Xu, Xin, Li, Yaxian, Wu, Youliang, Wang, Huizhen, Li, Yongxiang. 2022. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. In Redox biology, 59, 102564. doi:10.1016/j.redox.2022.102564. https://pubmed.ncbi.nlm.nih.gov/36473315/
2. Li, Yankang, Zhang, Nasha, Zhang, Li, Yu, Jinming, Yang, Ming. . Oncogene HSPH1 modulated by the rs2280059 genetic variant diminishes EGFR-TKIs efficiency in advanced lung adenocarcinoma. In Carcinogenesis, 41, 1195-1202. doi:10.1093/carcin/bgaa069. https://pubmed.ncbi.nlm.nih.gov/32815538/
3. Wu, Xinrui, Zhu, Yinjie, Hu, Cong, Dong, Liang, Pan, Jiahua. 2024. Extracellular vesicles related gene HSPH1 exerts anti-tumor effects in prostate cancer via promoting the stress response of CD8 + T cells. In Cellular oncology (Dordrecht, Netherlands), 47, 1059-1064. doi:10.1007/s13402-023-00905-7. https://pubmed.ncbi.nlm.nih.gov/38165608/
4. Song, Peng, Li, Xiang, Chen, Shuai, He, Jian, Tang, Liming. 2024. YTHDF1 mediates N-methyl-N-nitrosourea-induced gastric carcinogenesis by controlling HSPH1 translation. In Cell proliferation, 57, e13619. doi:10.1111/cpr.13619. https://pubmed.ncbi.nlm.nih.gov/38444279/
5. Robichaud, Sabrina, Fairman, Garrett, Vijithakumar, Viyashini, Baetz, Kristin, Ouimet, Mireille. 2021. Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells. In Autophagy, 17, 3671-3689. doi:10.1080/15548627.2021.1886839. https://pubmed.ncbi.nlm.nih.gov/33590792/
6. Zappasodi, Roberta, Ruggiero, Giusi, Guarnotta, Carla, Pupa, Serenella M, Di Nicola, Massimo. 2015. HSPH1 inhibition downregulates Bcl-6 and c-Myc and hampers the growth of human aggressive B-cell non-Hodgkin lymphoma. In Blood, 125, 1768-71. doi:10.1182/blood-2014-07-590034. https://pubmed.ncbi.nlm.nih.gov/25573990/
7. Liang, Yafeng, Luo, Jiaqi, Yang, Nengli, Ye, Mingwei, Pan, Guoquan. . Activation of the IL-1β/KLF2/HSPH1 pathway promotes STAT3 phosphorylation in alveolar macrophages during LPS-induced acute lung injury. In Bioscience reports, 40, . doi:10.1042/BSR20193572. https://pubmed.ncbi.nlm.nih.gov/32091104/
8. Franco, Juliana C, Nogueira, Maria L C, Gandelini, Gabriela M, Young, Jason C, Ramos, Carlos H I. . Sorghum bicolor SbHSP110 has an elongated shape and is able of protecting against aggregation and replacing human HSPH1/HSP110 in refolding and disaggregation assays. In Biopolymers, 114, e23532. doi:10.1002/bip.23532. https://pubmed.ncbi.nlm.nih.gov/36825649/
9. Qian, Hongyan, Qian, Yi, Liu, Yi, Liu, Huanxin, Zhu, Weizhong. 2023. Identification of novel biomarkers involved in doxorubicin-induced acute and chronic cardiotoxicity, respectively, by integrated bioinformatics. In Frontiers in cardiovascular medicine, 9, 996809. doi:10.3389/fcvm.2022.996809. https://pubmed.ncbi.nlm.nih.gov/36712272/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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