Slc6a4-flox Mouse
一般名
Slc6a4-flox
製品ID
S-CKO-03013
背景情報
C57BL/6JCya
系統ID
CKOCMP-15567-Slc6a4-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Slc6a4-flox Mouse（カタログ番号S-CKO-03013）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Slc6a4-flox
系統ID
CKOCMP-15567-Slc6a4-B6J-VA
遺伝子名
製品ID
S-CKO-03013
遺伝子別名
Htt, Sert, 5-HTT
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000021195
NCBIトランスクリプトID
NM_010484
ターゲット領域
Exon 3~4
有効領域の大きさ
~1.9 kb
遺伝子研究の概要
Slc6a4, encoding the serotonin transporter protein, is crucial for regulating serotonin (5-HT) levels, as the 5-HT transporter it codes for plays a key role in 5-HT reuptake [1,2,5]. Serotonin is involved in multiple physiological and psychological processes, and thus Slc6a4 is of great biological importance in relation to mental health and related functions [1,2,5].
Many studies focus on its polymorphisms and associations with various disorders. For example, the 5HTTLPR polymorphism in the 5' region of Slc6a4 has been widely studied in relation to psychiatric traits, with some suggesting associations with increased risk of posttraumatic stress disorder, higher likelihood for depression, obsessive-compulsive disorder, increased hostility and criminal behaviour [1,7]. Also, its DNA methylation has been associated with stress-related physiological changes; higher average levels of Slc6a4 DNAm were associated with higher average levels of salivary alpha-amylase (SAM axis) between-person, and with lower levels of salivary cortisol and alpha-amylase within-person [4]. In addition, in schizophrenia patients, the methylation level of Slc6a4 was significantly higher in women than in men, and higher in the schizophrenia group than in the control group, especially in the male subgroup [2]. However, meta-analysis did not find an association between Slc6a4 promoter and intron 2 variants and the risk of temporal lobe epilepsy, though the intron 2 VNTR seems to have opposite effects in different populations [3]. Also, no association was found between the Slc6a4 promoter polymorphism and alcohol dependence [6]. Moreover, the Slc6a4 short/short allele was found to increase anxiety symptoms in adolescents exposed to childhood food insecurity [8].
In conclusion, Slc6a4 is vital for regulating serotonin levels and is associated with numerous mental health-related conditions. Research on its polymorphisms, methylation, and gene-environment interactions has provided insights into the pathophysiology of various psychiatric and stress-related disorders, contributing to our understanding of the underlying mechanisms and potentially guiding future therapeutic strategies.
References：
1. Gelernter, Joel. 2014. SLC6A4 polymorphism, population genetics, and psychiatric traits. In Human genetics, 133, 459-61. doi:10.1007/s00439-013-1412-2. https://pubmed.ncbi.nlm.nih.gov/24385047/
2. Liu, Linlin, Hu, Yi, Lu, Yumei, Gao, Changqing, Nie, Shengjie. 2021. Sex-dependent DNA hypermethylation of SLC6A4 in patients with schizophrenia. In Neuroscience letters, 769, 136394. doi:10.1016/j.neulet.2021.136394. https://pubmed.ncbi.nlm.nih.gov/34910986/
3. Córdoba, Marta, Consalvo, Damián, Moron, Dolores Gonzalez, Kochen, Silvia, Kauffman, Marcelo Andrés. 2012. SLC6A4 gene variants and temporal lobe epilepsy susceptibility: a meta-analysis. In Molecular biology reports, 39, 10615-9. doi:10.1007/s11033-012-1949-5. https://pubmed.ncbi.nlm.nih.gov/23065262/
4. Stoffel, Martin, Rahn, Stefanie, Neubauer, Andreas B, Aguilar-Raab, Corina, Ditzen, Beate. 2023. Associations of SLC6A4 methylation with salivary cortisol, salivary alpha-amylase, and subjective stress in everyday life. In Psychoneuroendocrinology, 153, 106283. doi:10.1016/j.psyneuen.2023.106283. https://pubmed.ncbi.nlm.nih.gov/37196602/
5. Murphy, Dennis L, Moya, Pablo R. 2011. Human serotonin transporter gene (SLC6A4) variants: their contributions to understanding pharmacogenomic and other functional G×G and G×E differences in health and disease. In Current opinion in pharmacology, 11, 3-10. doi:10.1016/j.coph.2011.02.008. https://pubmed.ncbi.nlm.nih.gov/21439906/
6. Villalba, Karina, Attonito, Jennifer, Mendy, Angelico, Gasana, Janvier, Dorak, Tevfik M. . A meta-analysis of the associations between the SLC6A4 promoter polymorphism (5HTTLPR) and the risk for alcohol dependence. In Psychiatric genetics, 25, 47-58. doi:10.1097/YPG.0000000000000078. https://pubmed.ncbi.nlm.nih.gov/25710844/
7. Hande, Shyamala H, Krishna, Swathy M, Sahote, Komalroop Kaur, Ravidhran, Renuka, Das, Ranajit. . Population genetic variation of SLC6A4 gene, associated with neurophysiological development. In Journal of genetics, 100, . doi:. https://pubmed.ncbi.nlm.nih.gov/33764333/
8. Pilkay, S, Nolasco, M, Nunes, S, Trexler, N, Gump, B. . SLC6A4 gene variants moderate associations between childhood food insecurity and adolescent mental health. In Brain and behavior, 14, e3426. doi:10.1002/brb3.3426. https://pubmed.ncbi.nlm.nih.gov/38361316/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。