Itga2-flox Mouse
一般名
Itga2-flox
製品ID
S-CKO-03149
背景情報
C57BL/6JCya
系統ID
CKOCMP-16398-Itga2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Itga2-flox Mouse（カタログ番号S-CKO-03149）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Itga2-flox
系統ID
CKOCMP-16398-Itga2-B6J-VA
遺伝子名
製品ID
S-CKO-03149
遺伝子別名
DX5, GPIa, CD49B
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 13
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000056117
NCBIトランスクリプトID
NM_008396
ターゲット領域
Exon 2
有効領域の大きさ
~1.7 kb
遺伝子研究の概要
Itga2, also known as CD49b or VLA-2, is the alpha subunit of the transmembrane collagen receptor integrin alpha-2/beta-1. It is involved in cell adhesion, and its associated pathways may include those related to DNA damage response, immune regulation, and extracellular matrix-mediated cell-cell interactions. It plays an important role in various biological processes such as cell growth, invasion, and DNA repair, and is thus of great biological significance. Genetic models, like KO/CKO mouse models, can be valuable for studying its functions [1,5].
In pancreatic cancer, overexpression of Itga2 inhibits DNA repair by restraining the recruitment of DNA-PKcs to Ku70/80 heterodimer in the non-homologous end joining (NHEJ) pathway, conferring sensitivity to radiotherapy. It also affects immune regulation by influencing the expression of MET, PD-L1, CD4, and CD8, and induces STING expression by degrading DNMT1 [1,2,5]. In glioblastoma, Itga2 is a potential nanotherapeutic target as its antibody blockade impedes cell migration. In colorectal cancer, it enhances 5-fluorouracil resistance, and this can be overcome by selumetinib [3,4]. In esophageal squamous cell carcinoma, overexpression of Itga2 promotes tumor cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) via the FAK/AKT signaling pathway [6]. In intrahepatic cholangiocarcinoma, elevated Itga2 expression promotes collagen type I-induced clonogenic growth. In thyroid carcinoma, hypomethylation-mediated overexpression of Itga2 stimulates cell invasion and migration. In glioma, Itga2 promotes glioma stem cell (GSC) invasion and EMT by activating STAT3 phosphorylation [7,8,9].
In conclusion, Itga2 has diverse functions in multiple biological processes and disease conditions. Through model-based research, especially KO/CKO mouse models, it has been revealed that Itga2 is involved in DNA repair, immune regulation, cell growth, invasion, and migration in various cancers, providing potential therapeutic targets for these diseases.
References：
1. Zhou, Chen, Li, Shoukang, Bin, Kaijian, Wu, Heshui, Zhou, Yingke. 2022. ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer. In Cancer letters, 547, 215855. doi:10.1016/j.canlet.2022.215855. https://pubmed.ncbi.nlm.nih.gov/35998796/
2. Jin, Liquan, Duan, Yaoqiang, Li, Xiaoxi, Chen, Yiming, Tan, Yunbo. 2023. High expression ITGA2 affects the expression of MET, PD-L1, CD4 and CD8 with the immune microenvironment in pancreatic cancer patients. In Frontiers in immunology, 14, 1209367. doi:10.3389/fimmu.2023.1209367. https://pubmed.ncbi.nlm.nih.gov/37881431/
3. Guo, Peng, Moses-Gardner, Alexander, Huang, Jing, Smith, Edward R, Moses, Marsha A. 2019. ITGA2 as a potential nanotherapeutic target for glioblastoma. In Scientific reports, 9, 6195. doi:10.1038/s41598-019-42643-7. https://pubmed.ncbi.nlm.nih.gov/30996239/
4. Qin, Jian, Hu, Shangshang, Lou, Jinwei, Pan, Yuqin, Wang, Shukui. 2024. Selumetinib overcomes ITGA2-induced 5-fluorouracil resistance in colorectal cancer. In International immunopharmacology, 137, 112487. doi:10.1016/j.intimp.2024.112487. https://pubmed.ncbi.nlm.nih.gov/38889513/
5. Meng, Junpeng, Cai, Hongkun, Sun, Yan, Wu, Heshui, Ren, Dianyun. 2022. ITGA2 induces STING expression in pancreatic cancer by inducing DNMT1 degradation. In Cellular oncology (Dordrecht, Netherlands), 45, 1421-1434. doi:10.1007/s13402-022-00731-3. https://pubmed.ncbi.nlm.nih.gov/36331797/
6. Huang, Wei, Zhu, Ju, Shi, Haoming, Wu, Qingchen, Zhang, Cheng. 2021. ITGA2 Overexpression Promotes Esophageal Squamous Cell Carcinoma Aggression via FAK/AKT Signaling Pathway. In OncoTargets and therapy, 14, 3583-3596. doi:10.2147/OTT.S302028. https://pubmed.ncbi.nlm.nih.gov/34113124/
7. Rattanasinchai, Chotirat, Navasumrit, Panida, Ruchirawat, Mathuros. 2022. Elevated ITGA2 expression promotes collagen type I-induced clonogenic growth of intrahepatic cholangiocarcinoma. In Scientific reports, 12, 22429. doi:10.1038/s41598-022-26747-1. https://pubmed.ncbi.nlm.nih.gov/36575207/
8. Chen, Hong, Zhang, Chunying, Li, Yanbing, Chen, Chunyou. 2022. Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma. In Histology and histopathology, 38, 787-796. doi:10.14670/HH-18-552. https://pubmed.ncbi.nlm.nih.gov/36420922/
9. Zhang, Jin, Li, Ruinan, Zhang, Haibin, Wang, Shanshan, Zhao, Yuanli. . ITGA2 as a prognostic factor of glioma promotes GSCs invasion and EMT by activating STAT3 phosphorylation. In Carcinogenesis, 45, 235-246. doi:10.1093/carcin/bgad096. https://pubmed.ncbi.nlm.nih.gov/38142122/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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