Oprd1-flox Mouse
一般名
Oprd1-flox
製品ID
S-CKO-04117
背景情報
C57BL/6JCya
系統ID
CKOCMP-18386-Oprd1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Oprd1-flox Mouse（カタログ番号S-CKO-04117）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Oprd1-flox
系統ID
CKOCMP-18386-Oprd1-B6J-VA
遺伝子名
製品ID
S-CKO-04117
遺伝子別名
DOR, Nbor, mDOR, DOR-1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 4
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000056336
NCBIトランスクリプトID
NM_013622
ターゲット領域
Exon 2
有効領域の大きさ
~1.5 kb
遺伝子研究の概要
OPRD1 encodes the delta-opioid receptor, which has multiple functions including regulating reward pathways [1]. This receptor is a key component in the body's opioid-related physiological processes, and its associated pathways play a significant role in the body's response to various stimuli. Genetic models, such as gene knockout models, can be valuable in further exploring the gene's functions.
The OPRD1 gene contains over 2,000 verified genetic variants, with rs1042114 disrupting receptor maturation and rs569356 affecting its expression [1]. Polymorphisms in this gene have been associated with multiple human diseases. For instance, three variants in intron 1 (rs2236861, rs2236857, and rs3766951) are associated with opioid addiction in several independent populations [1]. A haplotype block containing rs569356 and rs533123 is associated with anorexia, though the mechanisms are unknown [1]. Rs1042114 has been linked to Alzheimer's disease, as the variant allele reduces production of beta-amyloid plaques [1]. Additionally, OPRD1 rs569356 affects plasma norbuprenorphine levels and craving/withdrawal symptoms in opioid-use-disorder patients [2]. Some OPRD1 intronic variants associated with opioid addiction are cis-eQTLs for PHACTR4, a gene involved in cytoskeletal dynamics [3]. In Han Chinese heroin-dependent patients, the rs2234918 minor C allele is over-represented in the HD group, and rs2236857 T/T homozygotes have more severe stress [4]. The rs678849 variant influences the outcome of disulfiram treatment for cocaine dependency in methadone-maintained patients [5]. And certain OPRD1 SNPs and a specific haplotype are associated with heroin dependence [6].
In conclusion, OPRD1 plays a crucial role in regulating reward pathways and is associated with various human diseases such as opioid addiction, anorexia, and Alzheimer's disease. Research using genetic models, especially when considering its genetic polymorphisms, can help in understanding its role in these disease conditions, potentially leading to new therapeutic strategies for these disorders.
References：
1. Crist, Richard C, Clarke, Toni-Kim. . OPRD1 Genetic Variation and Human Disease. In Handbook of experimental pharmacology, 247, 131-145. doi:10.1007/164_2016_112. https://pubmed.ncbi.nlm.nih.gov/28035534/
2. Kaya-Akyüzlü, Dilek, Özkan-Kotiloğlu, Selin, Danışman, Mustafa, Oğur, Begüm, İspir, Gamze Zengin. 2023. OPRD1 rs569356 polymorphism has an effect on plasma norbuprenorphine levels and dose/kg-normalized norbuprenorphine values in individuals with opioid use disorder. In Environmental toxicology and pharmacology, 100, 104143. doi:10.1016/j.etap.2023.104143. https://pubmed.ncbi.nlm.nih.gov/37146669/
3. Levran, Orna, Randesi, Matthew, Adelson, Miriam, Kreek, Mary Jeanne. 2021. OPRD1 SNPs associated with opioid addiction are cis-eQTLs for the phosphatase and actin regulator 4 gene, PHACTR4, a mediator of cytoskeletal dynamics. In Translational psychiatry, 11, 316. doi:10.1038/s41398-021-01439-y. https://pubmed.ncbi.nlm.nih.gov/34031368/
4. Huang, Chang-Chih, Kuo, Shin-Chang, Yeh, Ta-Chuan, Ho, Pei-Shen, Huang, San-Yuan. 2018. OPRD1 gene affects disease vulnerability and environmental stress in patients with heroin dependence in Han Chinese. In Progress in neuro-psychopharmacology & biological psychiatry, 89, 109-116. doi:10.1016/j.pnpbp.2018.08.028. https://pubmed.ncbi.nlm.nih.gov/30171993/
5. Thomas, Patrick S, Nielsen, Ellen M, Spellicy, Catherine J, Kosten, Thomas R, Nielsen, David A. . The OPRD1 rs678849 variant influences outcome of disulfiram treatment for cocaine dependency in methadone-maintained patients. In Psychiatric genetics, 31, 88-94. doi:10.1097/YPG.0000000000000279. https://pubmed.ncbi.nlm.nih.gov/33953123/
6. Nelson, Elliot C, Lynskey, Michael T, Heath, Andrew C, Martin, Nicholas G, Montgomery, Grant W. 2012. Association of OPRD1 polymorphisms with heroin dependence in a large case-control series. In Addiction biology, 19, 111-21. doi:10.1111/j.1369-1600.2012.00445.x. https://pubmed.ncbi.nlm.nih.gov/22500942/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。