Prkab1-flox Mouse
一般名
Prkab1-flox
製品ID
S-CKO-04435
背景情報
C57BL/6JCya
系統ID
CKOCMP-19079-Prkab1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Prkab1-flox Mouse（カタログ番号S-CKO-04435）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Prkab1-flox
系統ID
CKOCMP-19079-Prkab1-B6J-VA
遺伝子名
製品ID
S-CKO-04435
遺伝子別名
E430008F22, 1300015D22Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 5
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000031486
NCBIトランスクリプトID
NM_031869
ターゲット領域
Exon 2
有効領域の大きさ
~1.5 kb
遺伝子研究の概要
PRKAB1, encoding the β1 regulatory subunit of AMP-activated protein kinase (AMPK), is crucial in regulating the metabolism of carbohydrate, lipid, and protein, and is considered a key regulator of cellular energy homeostasis [2]. AMPK is an evolutionarily conserved sensor of cellular and systemic energy balance, and PRKAB1 has been shown to be highly involved in glycogen metabolism [7,8].
In a multi-omics study on Crohn's disease, PRKAB1 was identified as one of the genes involved in gene-microbiota interactions. The association between PRKAB1-Escherichia coli in the validation cohort was consistent with eQTL-mbQTL colocalization, suggesting its role in the disease mechanism [1]. In goats, four novel single nucleotide polymorphisms (SNPs) of PRKAB1 were found, with some SNPs showing significant associations with growth traits in Xuhuai goats [2]. In a Mendelian randomization study on glucose-lowering drug targets and gastrointestinal cancer, weak evidence indicated connections of PRKAB1 with anal carcinoma, cardia cancer, ICC, and rectum cancer [3]. A study on non-alcoholic steatohepatitis (NASH) showed that a compound, buddleoside, bound to the PRKAB1 subunit and activated AMPK, alleviating NASH symptoms [4]. Also, in non-alcoholic fatty liver disease (NAFLD), miR-802 was found to inhibit hepatic AMPK expression by binding to the 3' untranslated region of Prkab1, and schisanhenol improved NAFLD by targeting the miR-802/AMPK pathway related to PRKAB1 [5]. A Mendelian randomization study on lung cancer showed that the PRKAB1 gene was associated with a lower risk of developing lung adenocarcinoma [6].
In conclusion, PRKAB1 is essential for regulating cellular energy homeostasis and metabolism. Studies in various disease models such as Crohn's disease, NASH, NAFLD, and in relation to growth traits in goats, and cancer risks, have revealed its significance in disease mechanisms and biological processes. These model-based studies contribute to understanding the role of PRKAB1 in different physiological and pathological conditions.
References：
1. Xu, Shu, Li, Xiaozhi, Zhang, Shenghong, Feng, Rui, Chen, Minhu. 2023. Oxidative stress gene expression, DNA methylation, and gut microbiota interaction trigger Crohn's disease: a multi-omics Mendelian randomization study. In BMC medicine, 21, 179. doi:10.1186/s12916-023-02878-8. https://pubmed.ncbi.nlm.nih.gov/37170220/
2. Zhou, Shengliang, Shi, Xiuying, Song, Chengchuang, Chen, Hong, Fang, Xingtang. 2021. SNP discovery of PRKAB1 gene and their associations with growth traits in goats. In Animal biotechnology, 33, 1613-1619. doi:10.1080/10495398.2021.1920426. https://pubmed.ncbi.nlm.nih.gov/34106801/
3. Yang, Yi, Chen, Bo, Zheng, Chongming, Chen, Gang, Wang, Yi. 2024. Association of glucose-lowering drug target and risk of gastrointestinal cancer: a mendelian randomization study. In Cell & bioscience, 14, 36. doi:10.1186/s13578-024-01214-8. https://pubmed.ncbi.nlm.nih.gov/38504335/
4. Chen, Meng, Liu, Guowen, Fang, Zhiyuan, Du, Xiliang, Li, Xinwei. 2025. Buddleoside alleviates nonalcoholic steatohepatitis by targeting the AMPK-TFEB signaling pathway. In Autophagy, , 1-19. doi:10.1080/15548627.2025.2466145. https://pubmed.ncbi.nlm.nih.gov/39936600/
5. Li, Bin, Xiao, Qi, Zhao, Hongmei, Sun, Haitao, Liu, Haitao. 2024. Schisanhenol ameliorates non-alcoholic fatty liver disease via inhibiting miR-802 activation of AMPK-mediated modulation of hepatic lipid metabolism. In Acta pharmaceutica Sinica. B, 14, 3949-3963. doi:10.1016/j.apsb.2024.05.014. https://pubmed.ncbi.nlm.nih.gov/39309511/
6. Sun, Wen, Zhang, Xiaoyu, Li, Ning, Ji, Jianguang, Zheng, Deqiang. 2024. Genetic association of glycemic traits and antihyperglycemic agent target genes with the risk of lung cancer: A Mendelian randomization study. In Diabetes & metabolic syndrome, 18, 103048. doi:10.1016/j.dsx.2024.103048. https://pubmed.ncbi.nlm.nih.gov/38850595/
7. Zhang, Qin, Chen, Hong, Zhao, Sheng, Zhang, Liangzhi, Wang, Xueming. 2009. Polymorphisms in the promoter region of bovine PRKAB1 gene. In Molecular biology reports, 37, 435-40. doi:10.1007/s11033-009-9612-5. https://pubmed.ncbi.nlm.nih.gov/19609721/
8. Zhang, Qin, Chen, Hong, Zhao, Sheng, Li, Fei, Wang, Xueming. 2009. Single nucleotide polymorphisms and haplotypic diversity in the bovine PRKAB1 gene. In Molecular biotechnology, 43, 193-9. doi:10.1007/s12033-009-9194-4. https://pubmed.ncbi.nlm.nih.gov/19578998/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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