Rab3a-flox Mouse
一般名
Rab3a-flox
製品ID
S-CKO-04658
背景情報
C57BL/6JCya
系統ID
CKOCMP-19339-Rab3a-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Rab3a-flox Mouse（カタログ番号S-CKO-04658）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Rab3a-flox
系統ID
CKOCMP-19339-Rab3a-B6J-VA
遺伝子名
製品ID
S-CKO-04658
遺伝子別名
--
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 8
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000110093
NCBIトランスクリプトID
NM_001166399
ターゲット領域
Exon 2~4
有効領域の大きさ
~1.9 kb
遺伝子研究の概要
Rab3a, a small GTP-binding protein, is a member of the Rab family implicated in intracellular vesicle trafficking. It is crucial for Ca(2+)-dependent exocytosis, such as neurotransmitter release from nerve terminals [4]. Rab3a is also involved in various biological processes including plasma membrane repair, neurite outgrowth, and atrial natriuretic peptide secretion [1,2,3].
In plasma membrane repair, Rab3a is essential for lysosome exocytosis. It forms a tripartite complex with synaptotagmin-like protein 4a and non-muscle myosin heavy chain IIA, which is vital for positioning peripheral lysosomes for repair [1]. In hippocampal neurons, Rab3a interacts with spastin, regulating its protein stability and microtubule-severing function to modulate neurite outgrowth [2]. In cardiomyocytes, zDHHC9 palmitoylates Rab3gap1, influencing Rab3a activity and atrial natriuretic peptide secretion [3]. Silencing the Rab3A/Rab27A system in podocytes affects differentiation, apoptosis, and cytoskeletal organization, highlighting its role in podocyte injury [5]. In a rat spinal cord injury model, Rab3a promoted the secretion of neuronal cell-derived extracellular vesicles by interacting with STXBP1, which inhibited M1 macrophage polarization and thus promoted spinal cord injury repair [6]. In melanocytes, RAB3A regulates melanin exocytosis and transfer induced by keratinocyte-conditioned medium [7]. In murine leukemia virus infection, Rab3a is required for the stabilization of the Gag protein [8]. Heterozygous RAB3A variants, like p.Arg83Trp, cause cerebellar ataxia through a partial loss-of-function mechanism [9].
In summary, Rab3a plays essential roles in multiple biological processes including vesicle-related functions such as exocytosis, secretion, and membrane repair. Research using various models, including those with genetic manipulations, has revealed its significance in diseases like spinal cord injury, podocyte-related injury, and cerebellar ataxia, providing insights into potential therapeutic targets for these conditions.
References：
1. Vieira, Otilia V. 2016. Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair. In Small GTPases, 9, 349-351. doi:10.1080/21541248.2016.1235004. https://pubmed.ncbi.nlm.nih.gov/27687479/
2. Yang, Yuhao, Yang, Jie, Liang, Yaozhong, Lin, Hongsheng, Tan, Minghui. 2022. Rab3A interacts with spastin to regulate neurite outgrowth in hippocampal neurons. In Biochemical and biophysical research communications, 643, 77-87. doi:10.1016/j.bbrc.2022.12.066. https://pubmed.ncbi.nlm.nih.gov/36587525/
3. Essandoh, Kobina, Subramani, Arasakumar, Ferro, Olivia A, Koripella, Sribharat, Brody, Matthew J. 2023. zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1. In JACC. Basic to translational science, 8, 518-542. doi:10.1016/j.jacbts.2022.11.003. https://pubmed.ncbi.nlm.nih.gov/37325411/
4. Takai, Y, Sasaki, T, Shirataki, H, Nakanishi, H. . Rab3A small GTP-binding protein in Ca(2+)-dependent exocytosis. In Genes to cells : devoted to molecular & cellular mechanisms, 1, 615-32. doi:. https://pubmed.ncbi.nlm.nih.gov/9078389/
5. Martinez-Arroyo, Olga, Flores-Chova, Ana, Sanchez-Garcia, Belen, Cortes, Raquel, Ortega, Ana. 2023. Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes. In Biology, 12, . doi:10.3390/biology12050690. https://pubmed.ncbi.nlm.nih.gov/37237503/
6. Yang, Yuhao, Liu, Ziqiao, Lu, Yang, Wang, Zemin, Zha, Dingsheng. 2024. Rab3a attenuates spinal cord injury by mediating vesicle release. In Brain research bulletin, 208, 110884. doi:10.1016/j.brainresbull.2024.110884. https://pubmed.ncbi.nlm.nih.gov/38253132/
7. Cabaço, Luís C, Bento-Lopes, Liliana, Neto, Matilde V, Seabra, Miguel C, Barral, Duarte C. 2022. RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium. In JID innovations : skin science from molecules to population health, 2, 100139. doi:10.1016/j.xjidi.2022.100139. https://pubmed.ncbi.nlm.nih.gov/36090299/
8. Izumida, Mai, Kakoki, Katsura, Hayashi, Hideki, Matsuyama, Toshifumi, Kubo, Yoshinao. 2021. Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein. In Small GTPases, 13, 162-182. doi:10.1080/21541248.2021.1939631. https://pubmed.ncbi.nlm.nih.gov/34180342/
9. Hengel, Holger, Hannan, Shabab B, Reich, Selina, Schöls, Ludger, Synofzik, Matthis. 2025. Heterozygous RAB3A variants cause cerebellar ataxia by a partial loss-of-function mechanism. In Brain : a journal of neurology, , . doi:10.1093/brain/awaf111. https://pubmed.ncbi.nlm.nih.gov/40166812/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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