Tcf7-flox Mouse
一般名
Tcf7-flox
製品ID
S-CKO-05804
背景情報
C57BL/6JCya
系統ID
CKOCMP-21414-Tcf7-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Tcf7-flox Mouse（カタログ番号S-CKO-05804）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Tcf7-flox
系統ID
CKOCMP-21414-Tcf7-B6J-VA
遺伝子名
製品ID
S-CKO-05804
遺伝子別名
TCF-1, Tcf1
遺伝子別名
C57BL/6JCya
遺伝子正式名称
transcription factor 7, T cell specific
NCBI ID
修正
Conditional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000086844
NCBIトランスクリプトID
NM_009331
ターゲット領域
Exon 3
有効領域の大きさ
~1.5 kb
遺伝子研究の概要
Tcf7, also known as Transcription Factor 7, is a gene expressed in immune cells and is involved in multiple biological processes. It is the key downstream functional molecule of the Wnt/β -catenin signaling pathway, which participates in regulating various cellular activities [7].
In cancer research, TCF7-positive T cells in oral cancer are associated with tertiary lymphoid structures/organs and a superior prognosis. These cells express high levels of TLS-related genes and low levels of immune checkpoint molecules, suggesting they could be a new therapeutic target and prognostic marker [1]. In melanoma, the visualization of TCF7 within CD8+ T cells in fixed tumor samples predicted positive clinical outcome in checkpoint-treated patients [4]. In colorectal cancer, Dihydroartemisinin inhibits cancer development through the GSK-3β/TCF7/MMP9 pathway [6]. Also, in the context of ligamentum flavum hypertrophy, TCF7 promotes the hyper-proliferation and fibrosis phenotype through the TCF7/SNAI2/miR-4306 feedback loop, and its inhibition could be a novel therapeutic approach [7].
In CD8+ T cell research, Tcf7hi effector cells can give rise to central memory CD8+ T cells, and Tcf1 (a product of Tcf7) counteracts the differentiation of these cells [2]. Additionally, a microbial metabolite, indole-3-propionic acid (IPA), enhances immunotherapy efficacy by modulating T cell stemness through increasing H3K27 acetylation at the super-enhancer region of Tcf7 [3].
In the study of glucose homeostasis, Tcf7 expression is not a critical determinant in mice, and its expression in islet mRNA is due to islet-associated murine immune cells rather than islet β-cells [5].
In conclusion, Tcf7 is involved in multiple biological processes, especially in immune-related functions and cancer-associated mechanisms. Research using various models, though not always KO/CKO mouse models, has revealed its importance in cancer prognosis, immunotherapy response, and cell-fate determination in T cells. Its role in non-cancerous conditions like glucose homeostasis seems to be negligible. The study of Tcf7 provides valuable insights into immune-mediated diseases and cancer immunotherapy [1-7].
References：
1. Peng, Yu, Xiao, Liping, Rong, Haixu, Fan, Song, Li, Jinsong. 2021. Single-cell profiling of tumor-infiltrating TCF1/TCF7+ T cells reveals a T lymphocyte subset associated with tertiary lymphoid structures/organs and a superior prognosis in oral cancer. In Oral oncology, 119, 105348. doi:10.1016/j.oraloncology.2021.105348. https://pubmed.ncbi.nlm.nih.gov/34044317/
2. Pais Ferreira, Daniela, Silva, Joana Gomes, Wyss, Tania, Speiser, Daniel E, Held, Werner. 2020. Central memory CD8+ T cells derive from stem-like Tcf7hi effector cells in the absence of cytotoxic differentiation. In Immunity, 53, 985-1000.e11. doi:10.1016/j.immuni.2020.09.005. https://pubmed.ncbi.nlm.nih.gov/33128876/
3. Jia, Dingjiacheng, Wang, Qiwen, Qi, Yadong, Chen, Shujie, Wang, Liangjing. 2024. Microbial metabolite enhances immunotherapy efficacy by modulating T cell stemness in pan-cancer. In Cell, 187, 1651-1665.e21. doi:10.1016/j.cell.2024.02.022. https://pubmed.ncbi.nlm.nih.gov/38490195/
4. Sade-Feldman, Moshe, Yizhak, Keren, Bjorgaard, Stacey L, Getz, Gad, Hacohen, Nir. . Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. In Cell, 175, 998-1013.e20. doi:10.1016/j.cell.2018.10.038. https://pubmed.ncbi.nlm.nih.gov/30388456/
5. Kaur, Kiran Deep, Wong, Chi Kin, Baggio, Laurie L, Cao, Xiemin, Drucker, Daniel J. 2021. TCF7 is not essential for glucose homeostasis in mice. In Molecular metabolism, 48, 101213. doi:10.1016/j.molmet.2021.101213. https://pubmed.ncbi.nlm.nih.gov/33741532/
6. Dai, Xiaoshuo, Chen, Wei, Qiao, Yan, Dong, Ziming, Lu, Jing. 2023. Dihydroartemisinin inhibits the development of colorectal cancer by GSK-3β/TCF7/MMP9 pathway and synergies with capecitabine. In Cancer letters, 582, 216596. doi:10.1016/j.canlet.2023.216596. https://pubmed.ncbi.nlm.nih.gov/38101610/
7. Duan, Yang, Li, Jianjun, Qiu, Sujun, Ni, Songjia, Cao, Yanlin. 2022. TCF7/SNAI2/miR-4306 feedback loop promotes hypertrophy of ligamentum flavum. In Journal of translational medicine, 20, 468. doi:10.1186/s12967-022-03677-0. https://pubmed.ncbi.nlm.nih.gov/36224570/
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